[en] OBJECTIVE: The Wnt/beta-catenin signaling pathway has been implicated in human heart valve disease and is required for early heart valve formation in mouse and zebrafish. However, the specific functions of Wnt/beta-catenin signaling activity in heart valve maturation and maintenance in adults have not been determined previously. APPROACH AND RESULTS: Here, we show that Wnt/beta-catenin signaling inhibits Sox9 nuclear localization and proteoglycan expression in cultured chicken embryo aortic valves. Loss of beta-catenin in vivo in mice, using Periostin(Postn)Cre-mediated tissue-restricted loss of beta-catenin (Ctnnb1) in valvular interstitial cells, leads to the formation of aberrant chondrogenic nodules and induction of chondrogenic gene expression in adult aortic valves. These nodular cells strongly express nuclear Sox9 and Sox9 downstream chondrogenic extracellular matrix genes, including Aggrecan, Col2a1, and Col10a1. Excessive chondrogenic proteoglycan accumulation and disruption of stratified extracellular matrix maintenance in the aortic valve leaflets are characteristics of myxomatous valve disease. Both in vitro and in vivo data demonstrate that the loss of Wnt/beta-catenin signaling leads to increased nuclear expression of Sox9 concomitant with induced expression of chondrogenic extracellular matrix proteins. CONCLUSIONS: beta-Catenin limits Sox9 nuclear localization and inhibits chondrogenic differentiation during valve development and in adult aortic valve homeostasis.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fang, Ming
Alfieri, Christina M.
Hulin, Alexia ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : GIGA - Cardiovascular Sciences
Conway, Simon J.
Yutzey, Katherine E.
Language :
English
Title :
Loss of beta-catenin promotes chondrogenic differentiation of aortic valve interstitial cells.
Publication date :
2014
Journal title :
Arteriosclerosis, Thrombosis and Vascular Biology
ISSN :
1079-5642
eISSN :
1524-4636
Publisher :
Lippincott Williams and Wilkins, Philadelphia, United States - Pennsylvania
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