Article (Scientific journals)
Endometrial gene expression profiling in pregnant Meishan and Yorkshire pigs on day 12 of gestation
Gu, T.; Zhu, M.-J.; Schroyen, Martine et al.
2014In BMC Genomics, 15 (1)
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Keywords :
Differentially expressed genes; Endometrium; Implantation; Pig; Prolificacy; Meishan pig; Yorkshire pig; Suidae; Sus; Animals; Cluster Analysis; Computational Biology; Female; Gene Expression Profiling; Gene Expression Regulation; Gene Regulatory Networks; Litter Size; Molecular Sequence Annotation; Pregnancy; Reproducibility of Results; Swine; Transcriptome
Abstract :
[en] Background: Litter size in pigs is a major factor affecting the profitability in the pig industry. The peri-implantation window in pigs is characterized by the coordinated interactions between the maternal uterine endometrium and the rapidly elongating conceptuses and represents a period of time during which a large percentage of the developing conceptuses are lost. However, the gene expression and regulatory networks in the endometrium contributing to the establishment of the maternal: placental interface remain poorly understood.Results: We characterized the endometrial gene expression profile during the peri-implantation stage of development by comparing two breeds that demonstrate very different reproductive efficiencies. We employed the porcine Affymetrix GeneChip® to assay the transcriptomic profiles of genes expressed in the uterine endometrium obtained from Meishan and Yorkshire gilts (n = 4 for each breed) on day 12 of gestation (M12 and Y12, respectively). Total of 17,076 probesets were identified as "present" in at least two arrays. A mixed model-based statistical analysis predicted a total of 2,656 (q < 0.1) transcripts as differentially expressed between Meishan and Yorkshire pigs. Eighteen differentially expressed transcripts of interest were validated by quantitative real-time PCR. Gene ontology (GO) annotation revealed that the known functions of the differentially expressed genes were involved in a series of important biological processes relevant to early pregnancy establishment in the pig.Conclusions: The results identified endometrial gene expression profiles of two breeds differing in litter size and identified candidate genes that are related to known physiological pathways related to reproductive prolificacy. These findings provide a deeper understanding of molecular pathways differing between two breeds at the critical peri-implantation stage of pregnancy, which can be utilized to better understand the events contributing to pregnancy establishment in the pig. © 2014 Gu et al.; licensee BioMed Central Ltd.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Gu, T.;  Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
Zhu, M.-J.;  Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
Schroyen, Martine  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Zootechnie
Qu, L.;  Department of Statistics, Iowa State University, Ames 50011, United States
Nettleton, D.;  Department of Statistics, Iowa State University, Ames 50011, United States
Kuhar, D.;  Animal Parasitic Diseases Laboratory, BARC, ARS, USDA, Beltsville, MD 20705, United States
Lunney, J. K.;  Animal Parasitic Diseases Laboratory, BARC, ARS, USDA, Beltsville, MD 20705, United States
Ross, J. W.;  Department of Animal Science, Iowa State University, Ames 50011, United States
Zhao, S.-H.;  Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China, Department of Animal Science, Iowa State University, Ames 50011, United States
Tuggle, C. K.;  Department of Animal Science, Iowa State University, Ames 50011, United States
Title :
Endometrial gene expression profiling in pregnant Meishan and Yorkshire pigs on day 12 of gestation
Publication date :
2014
Journal title :
BMC Genomics
eISSN :
1471-2164
Publisher :
BioMed Central
Volume :
15
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NRI 2004-35205-14202, USDA, U.S. Department of Agriculture
Available on ORBi :
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