VIII. Analyse chimique et électrotitrimétrique de l'acide ribonucléique de la levure

The fractions of different molecular weights of yeast ribonucleic acid have been analyzed by chromatography and by the electrotitration curves. The results obtained by these two methods are practically the same if we assume that there is 0.65 secondary phosphoric acid group per four purine and pyrimidine bases. The chemical composition of all the fractions obtained by fractional-extraction, is practically the same, so that there must be a statistical distribution of the bases in the high molecular weight ribonucleic acid. The depolymerisatin to very low molecular weight degradation products docs not seem to liberate free secondary phosphoric acid groups. Il appears that in a "native" yeast ribonucleic acid, the molecular proportions of the four bases are nearly identical.