Article (Scientific journals)
Crystal structure and biochemical characterization of the transmembrane PAP2 type phosphatidylglycerol phosphate phosphatase from Bacillus subtilis.
El Ghachi, Meriem; Howe, Nicole; Auger, Rodolphe et al.
2017In Cellular and Molecular Life Sciences
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Keywords :
Bacterial lipids metabolism; Membrane protein structure; Peptidoglycan-related lipid; Undecaprenyl phosphate
Abstract :
[en] Type 2 phosphatidic acid phosphatases (PAP2s) can be either soluble or integral membrane enzymes. In bacteria, integral membrane PAP2s play major roles in the metabolisms of glycerophospholipids, undecaprenyl-phosphate (C55-P) lipid carrier and lipopolysaccharides. By in vivo functional experiments and biochemical characterization we show that the membrane PAP2 coded by the Bacillus subtilis yodM gene is the principal phosphatidylglycerol phosphate (PGP) phosphatase of B. subtilis. We also confirm that this enzyme, renamed bsPgpB, has a weaker activity on C55-PP. Moreover, we solved the crystal structure of bsPgpB at 2.25 A resolution, with tungstate (a phosphate analog) in the active site. The structure reveals two lipid chains in the active site vicinity, allowing for PGP substrate modeling and molecular dynamic simulation. Site-directed mutagenesis confirmed the residues important for substrate specificity, providing a basis for predicting the lipids preferentially dephosphorylated by membrane PAP2s.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
El Ghachi, Meriem ;  Université de Liège > Département des sciences de la vie > Cristallographie des macromolécules biologiques
Howe, Nicole
Auger, Rodolphe
Lambion, Alexandre
Guiseppi, Annick
Delbrassine, François 
Manat, Guillaume
Roure, Sophie
Peslier, Sabine
Sauvage, Eric ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Vogeley, Lutz
Rengifo-Gonzalez, Juan-Carlos
Charlier, Paulette ;  Université de Liège > Département des sciences de la vie > Cristallographie des macromolécules biologiques
Mengin-Lecreulx, Dominique
Foglino, Maryline
Touze, Thierry
Caffrey, Martin
Kerff, Frédéric  ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
More authors (8 more) Less
Language :
English
Title :
Crystal structure and biochemical characterization of the transmembrane PAP2 type phosphatidylglycerol phosphate phosphatase from Bacillus subtilis.
Publication date :
06 February 2017
Journal title :
Cellular and Molecular Life Sciences
ISSN :
1420-682X
eISSN :
1420-9071
Publisher :
Birkhauser Verlag, Switzerland
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 March 2017

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