[en] Enzymatic hydrolysis of wheat gluten protein improves its solubility and produces hydrolysates withfoaming properties which may find applications in food products. First, we here investigated whetherfoam-liquid fractionation can concentrate wheat gluten peptides with foaming properties. Foam andliquid fractions had high and very low foam stability (FS), respectively. In addition, foam fractions wereable to decrease surface tension more pronouncedly than un-fractionated samples and liquid fractions,suggesting they are able to arrange themselves more efficiently at an interface. As a second objective,foam fractionation served as a tool to study the structural properties of the peptides, causing thesedifferences in air-water interfacial behavior. Zeta potential and surface hydrophobicity measurements didnot fully explain these differences but suggested that hydrophobic interactions at the air-water interfaceare more important than electrostatic interactions. RP-HPLC showed a large overlap between foam andliquid fractions. However, a small fraction of very hydrophobic peptides with relatively high averagemolecular mass was clearly enriched in the foam fraction. These peptides were also more concentratedin un-fractionated DH 2 hydrolysates, which had high FS, than in DH 6 hydrolysates, which had low FS.These peptides most likely play a key role in stabilizing the air-water interface.
Disciplines :
Food science
Author, co-author :
Wouters, Arno G.B.
Rombouts, Ine
Schoebrechts, Nele
Fierens, Ellen
Brijs, Kristof
Blecker, Christophe ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Science des alim. et formul.
Delcour, Jan A.
Language :
English
Title :
Foam fractionation as a tool to study the air-water interfacestructure-function relationship of wheat gluten hydrolysates
[1] Van Der Borght, A., Goesaert, H., Veraverbeke, W.S., Delcour, J.A., Fractionation of wheat and wheat flour into starch and gluten: overview of the main processes and the factors involved. J. Cereal Sci. 41 (2005), 221–237.
[3] Veraverbeke, W.S., Delcour, J.A., Wheat protein composition and properties of wheat glutenin in relation to breadmaking functionality. Crit. Rev. Food Sci. Nutr. 42 (2002), 179–208.
[4] Delcour, J.A., Joye, I.J., Pareyt, B., Wilderjans, E., Brijs, K., Lagrain, B., Wheat gluten functionality as a quality determinant in cereal-based food products. Annu. Rev. Food Sci. Technol. 3 (2012), 469–492.
[5] Adler-Nissen, J., Enzymatic hydrolysis of proteins for increased solubility. J. Agric. Food Chem. 24 (1976), 1090–1093.
[6] Damodaran, S., Protein stabilization of emulsions and foams. J. Food Sci. 70 (2005), R54–R66.
[7] Murray, B.S., Stabilization of bubbles and foams. Curr. Opin. Colloid Interface Sci. 12 (2007), 232–241.
[8] Hunter, T.N., Pugh, R.J., Franks, G.V., Jameson, G.J., The role of particles in stabilising foams and emulsions. Adv. Colloid Interface Sci. 137 (2008), 57–81.
[11] Linares, E., Larre, C., Lemeste, M., Popineau, Y., Emulsifying and foaming properties of gluten hydrolysates with an increasing degree of hydrolysis: role of soluble and insoluble fractions. Cereal Chem. 77 (2000), 414–420.
[12] Mimouni, B., Raymond, J., Merle-Desnoyers, A.M., Azanza, J.L., Ducastaing, A., Combined acid deamidation and enzymic hydrolysis for improvement of the functional properties of wheat gluten. J. Cereal Sci. 20 (1994), 153–165.
[13] Wouters, A.G.B., Rombouts, I., Legein, M., Fierens, E., Brijs, K., Blecker, C., Delcour, J.A., Air–water interfacial properties of enzymatic wheat gluten hydrolyzates determine their foaming behavior. Food Hydrocolloid 55 (2016), 155–162.
[14] Agyare, K.K., Addo, K., Xiong, Y.L., Emulsifying and foaming properties of transglutaminase-treated wheat gluten hydrolysate as influenced by ph, temperature and salt. Food Hydrocolloid 23 (2009), 72–81.
[15] Babiker, E.E., Fujisawa, N., Matsudomi, N., Kato, A., Improvement in the functional properties of gluten by protease digestion or acid hydrolysis followed by microbial transglutaminase treatment. J. Agric. Food Chem. 44 (1996), 3746–3750.
[16] Berot, S., Popineau, Y., Compoint, J.P., Blassel, C., Chaufer, B., Ultrafiltration to fractionate wheat polypeptides. J. Chromatogr. B 753 (2001), 29–35.
[17] Popineau, Y., Huchet, B., Larre, C., Berot, S., Foaming and emulsifying properties of fractions of gluten peptides obtained by limited enzymatic hydrolysis and ultrafiltration. J. Cereal Sci. 35 (2002), 327–335.
[18] Wang, J.S., Zhao, M.M., Bao, Y., Hong, T., Rosella, C.M., Preparation and characterization of modified wheat gluten by enzymatic hydrolysis-ultrafiltration. J. Food Biochem. 32 (2008), 316–334.
[19] Wang, J.S., Zhao, M.M., Yang, X.Q., Jiang, Y.M., Improvement on functional properties of wheat gluten by enzymatic hydrolysis and ultrafiltration. J. Cereal Sci. 44 (2006), 93–100.
[20] Barackov, I., Mause, A., Kapoor, S., Winter, R., Schembecker, G., Burghoff, B., Investigation of structural changes of β-casein and lysozyme at the gas–liquid interface during foam fractionation. J. Biotechnol. 161 (2012), 138–146.
[22] Shea, A.P., Crofcheck, C.L., Payne, F.A., Xiong, Y.L., Foam fractionation of α-lactalbumin and β-lactoglobulin from a whey solution. Asia-Pac. J. Chem. Eng. 4 (2009), 191–203.
[23] Lockwood, C.E., Bummer, P.M., Jay, M., Purification of proteins using foam fractionation. Pharm. Res. 14 (1997), 1511–1515.
[24] Nakabayashi, T., Takakusagi, Y., Iwabata, K., Sakaguchi, K., Foam fractionation of protein: correlation of protein adsorption onto bubbles with a ph-induced conformational transition. Anal. Biochem. 419 (2011), 173–179.
[25] Dhordain, P., Bigan, M., Vanhoute, M., Pierlot, C., Aubry, J.M., Dhulster, P., Guillochon, D., Froidevaux, R., Optimization of peptide separation from complex peptide mixture in a foaming-draining system. Sep. Sci. Technol. 47 (2012), 654–662.
[26] Vanhoute, M., Froidevaux, R., Pierlot, C., Krier, F., Aubry, J.M., Guillochon, D., Advancement of foam separation of bioactive peptides using an aeration column with a bubbling-draining method. Sep. Purif. Technol. 63 (2008), 460–465.
[27] AOAC, Official Methods of Analysis. Method 990.03. 1995, Association of Official Analytical Chemists, Washington, DC, USA.
[28] Adler-Nissen, J., Enzymic Hydrolysis of Food Proteins. 1985, Elsevier Applied Science Publishers, New York, USA p.
[29] Diermayr, P., Dehne, L., Controlled enzymatic hydrolysis of proteins at low ph values. 1. Experiments with bovine serum-albumin. Z. Lebensm. Unters. Forsch. 190 (1990), 516–520.
[30] Nielsen, P.M., Petersen, D., Dambmann, C., Improved method for determining food protein degree of hydrolysis. J. Food Sci. 66 (2001), 642–646.
[31] Caessens, P.W.J.R., Gruppen, H., Visser, S., van Aken, G.A., Voragen, A.G.J., Plasmin hydrolysis of beta-casein: foaming and emulsifying properties of the fractionated hydrolysate. J. Agric. Food Chem. 45 (1997), 2935–2941.
[32] Celus, I., Brijs, K., Delcour, J.A., Enzymatic hydrolysis of brewers' spent grain proteins and technofunctional properties of the resulting hydrolysates. J. Agric. Food Chem. 55 (2007), 8703–8710.
[33] Guan, X., Yao, H.Y., Chen, Z.X., Shan, L.A., Zhang, M.D., Some functional properties of oat bran protein concentrate modified by trypsin. Food Chem. 101 (2007), 163–170.
[34] Wouters, A.G.B., Rombouts, I., Fierens, E., Brijs, K., Delcour, J.A., Relevance of the functional properties of enzymatic plant protein hydrolysates in food systems. Comp. Rev. Food Sci. Food Saf. 15 (2016), 786–800.
[35] Murray, B.S., Rheological properties of protein films. Curr. Opin. in Colloid & Interface Sci. 16 (2011), 27–35.