Reference : Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet ac...
Scientific journals : Article
Human health sciences : Hematology
http://hdl.handle.net/2268/208095
Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides
English
Delierneux, Céline [Université de Liège - ULiège > > > Doct. sc. bioméd. & pharma. (Bologne)]
Donis, Nathalie [Université de Liège > > GIGA-Research >]
servais, laurence [Université de Liège - ULiège > GIGA-Sciences cardiovasculaires > > >]
Wéra, Odile [Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : GIGA - Cardiovascular Sciences >]
LECUT, Christelle [Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie biologique et immuno-hématologie >]
Vandereyken, Maud [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Musumeci, Lucia [Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : GIGA - Cardiovascular Sciences >]
Rahmouni, Souad [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Schneider, jochen [University of Luxembourg - UL > Luxembourg Centre for Systems Biomedicine > > >]
Eble, johannes [University of Münster > Institute for Physiological Chemistry and Pathobiochemistry > > >]
LANCELLOTTI, Patrizio [Centre Hospitalier Universitaire de Liège - CHU > > Service de cardiologie >]
Oury, Cécile mailto [Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : GIGA - Cardiovascular Sciences >]
May-2017
Journal of Thrombosis and Haemostasis
Blackwell Publishing
15
5
983-997
Yes (verified by ORBi)
International
1538-7933
1538-7836
Oxford
United Kingdom
[en] immunotherapy ; platelets
[en] Background: Synthetic phosphorothioate-modified CpG oligodeoxynucleotides (CpG ODNs) display potent immunostimulatory properties that are widely exploited in clinical trials of anticancer treatment. Unexpectedly, a recent study indicates that CpG ODNs activate human platelets via the immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptor glycoprotein VI.
Objective: To further analyze the mechanisms of CpG ODN-induced platelet activation and identify potential inhibitory strategies.
Methods: In vitro analyses were performed on human and mouse platelets, and on cell lines expressing platelet ITAM receptors. CpG ODN platelet activating effects were evaluated in a mouse model of thrombosis.
Results: We demonstrated platelet uptake of CpG ODNs, resulting in platelet activation and aggregation. The C-type lectin-like receptor 2 (CLEC-2) expressed in DT40 cells bound CpG ODNs. CpG ODN uptake did not occur in CLEC-2-deficient mouse platelets. Inhibition of human CLEC-2 with a blocking antibody inhibited CpG ODN-induced platelet aggregation. CpG ODNs caused CLEC-2 dimerization, and provoked its internalization. They induced dense granule release before the onset of aggregation. Accordingly, pretreating platelets with apyrase, or inhibiting P2Y12 with cangrelor or clopidogrel prevented CpG ODN platelet activating effect. In vivo, intravenously injected CpG ODN interacted with platelets adhered to mouse injured endothelium, and promoted thrombus growth, which was inhibited by CLEC-2 deficiency or by clopidogrel.
Conclusions: CLEC-2 and P2Y12 are required for CpG ODN-induced platelet activation and thrombosis and might be targeted to prevent adverse events in patients at risk.
http://hdl.handle.net/2268/208095
10.1111/jth.13669

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