eCG; PMSG; repeated high dose; cows; Ovum pick up; refractoriness
Abstract :
[en] This study performed plasma eCG binding rates assay on 8 cows submitted to repeated and high doses eCG administration in an ovum pick-up experimental protocol (de Roover et al., Abs.). The cows were divided in two groups. For each cow, blood samples were taken three times a week, before and during the period of treatments. Cows of the first group received 10 injections of eCG (2000UI; 4x1000 UI; 5x2000 UI) while cows of the second group received 5 eCG treatments (2000 UI).Statistical analysis of results was made using the "SAS General Linear Model Procedure (GLM)" with binding rate considered as dependent variable and animals, treatment, and stade (before or after treatment) as independent variables. A significative (p<0.01) difference was found between binding rates before and after the treatment in either the first or the second group. The two treatments were found to induce a significative (p<0.001) increase in binding rates when compared with values obtained before treatments. Even if all cows responded to treatments by a binding rate increase, the effect of the treatment differed significantly (p<0.01) from one cow to another which could be explained by a large individual variability.
From this experiment, we conclude that production of eCG antibodies is a wide response in the cows herein presented and repeatedly submitted to high doses of eCG. This immune response appeared after three to five injections and binding rates remained elevated over weeks following the latest injection. Special care should be given to this active immunisation -that could explain refractoriness to treatments- if such repeated administrations high doses of eCG were applied in usual protocols.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Drion, Pierre ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Méth. expér. des anim. de labo et éthique en expér. animale
De Roover, R.
Houtain JY
Remy, Benoit
Sulon, José
Dessy, F.
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Repeated high doses administration of eCG in cows submitted to an ovum pick-up experimental protocol: plasmatic eCG binding rate follow up
Publication date :
12 September 1997
Event name :
13è Colloque Association Européenne de Transfert Embryonnaire
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.