Biocompatibility of polymer-infiltrated-ceramicnetwork (PICN) materials with Human Gingival Keratinocytes (HGKs)

Biocompatibility; Polymer infiltrated ceramic network; CAD–CAM composite; Lithium disilicate glass-ceramic; Zirconia; Titanium; Human Gingival Keratinocytes; Monomer release; Cytotoxicity; Dental implant prostheses

Abstract :

[en] Objective. Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials, a new class of CAD–CAM composites, is poorly explored in the literature, in particular, no data are available regarding Human Gingival Keratinocytes (HGK). The first objective of this study was to evaluate the in vitro biocompatibility of PICNs with HGKs in comparison with other materials typically used for implant prostheses. The second objective was to correlate results with PICN monomer release and indirect cytotoxicity. Methods. HGK attachment, proliferation and spreading on PICN, grade V titanium (Ti), yttrium zirconia (Zi), lithium disilicate glass-ceramic (eM) and polytetrafluoroethylene (negative control) discs were evaluated using a specific insert-based culture system. For PICN and eM samples, monomer release in the culture medium was quantified by high performance liquid chromatography and indirect cytotoxicity tests were performed. Results. Ti and Zi exhibited the best results regarding HGK viability, number and coverage. eM showed inferior results while PICN showed statistically similar results to eM but also to Ti regarding cell number and to Ti and Zi regarding cell viability. No monomer release from PICN discs was found, nor indirect cytotoxicity, as for eM. Significance. The results confirmed the excellent behavior of Ti and Zi with gingival cells. Even if polymer based, PICN materials exhibited intermediate results between Ti–Zi and eM. These promising results could notably be explained by PICN high temperature–high pressure (HT–HP) innovative polymerization mode, as confirmed by the absence of monomer release and indirect cytotoxicity

Research Center/Unit :

d‐BRU - Dental Biomaterials Research Unit - ULiège

Disciplines :

Dentistry & oral medicine

Author, co-author :

GRENADE, Charlotte ; Centre Hospitalier Universitaire de Liège - CHU > Service prothèse fixée

De Pauw-Gillet, Marie-Claire ; Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

PIRARD, Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Service de toxicologie

Bertrand, Virginie ; Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)

Charlier, Corinne ; Université de Liège > Département de pharmacie > Chimie toxicologique

VAN HEUSDEN, Alain ; Centre Hospitalier Universitaire de Liège - CHU > Service prothèse fixée

Mainjot, Amélie ; Université de Liège > Département de sciences dentaires > Biomatériaux dentaires

Language :

English

Title :

Biocompatibility of polymer-infiltrated-ceramicnetwork (PICN) materials with Human Gingival Keratinocytes (HGKs)

Publication date :

09 January 2017

Journal title :

Dental Materials

ISSN :

0109-5641

eISSN :

1879-0097

Publisher :

Elsevier Science, Kidlington, United Kingdom

Volume :

Pages :

333-343

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 February 2017

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12 (10 by ULiège)

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Bibliography

[1] Abrahamsson, I., Berglundh, T., Glantz, P.O., Lindhe, J., The mucosal attachment at different abutments. An experimental study in dogs. J Clin Periodontol 25 (1998), 721–727.
[2] Abrahamsson, I., Berglundh, T., Lindhe, J., The mucosal barrier following abutment dis/reconnection. An experimental study in dogs. J Clin Periodontol 24 (1997), 568–572.
[3] Rompen, E., The impact of the type and configuration of abutments and their (repeated) removal on the attachment level and marginal bone. Eur J Oral Implantol 5:Suppl (2012), S83–S90.
[4] Mainjot, A.K., Dupont, N.M., Oudkerk, J.C., Dewael, T.Y., Sadoun, M.J., From artisanal to CAD-CAM blocks: state of the art of indirect composites. J Dent Res 95 (2016), 487–495.
[5] Swain, M.V., Coldea, A., Bilkhair, A., Guess, P.C., Interpenetrating network ceramic-resin composite dental restorative materials. Dent Mater 32 (2016), 34–42.
[6] Sadoun M. Inventor. Composite ceramic block. US patent 8,507,578 B2; 2011.
[7] Nguyen, J.-F., Migonney, V., Ruse, N.D., Sadoun, M., Resin composite blocks via high-pressure high-temperature polymerization. Dent Mater 28 (2012), 529–534.
[8] Nguyen, J.F., Ruse, D., Phan, A.C., Sadoun, M.J., High-temperature-pressure polymerized resin-infiltrated ceramic networks. J Dent Res 93 (2014), 62–67.
[9] Phan, A.C., Tang, M.L., Nguyen, J.-F., Ruse, N.D., Sadoun, M., High-temperature high-pressure polymerized urethane dimethacrylate—mechanical properties and monomer release. Dent Mater 30 (2014), 350–356.
[10] Phan, A.C., Béhin, P., Stoclet, G., Dorin Ruse, N., Nguyen, J.-F., Sadoun, M., Optimum pressure for the high-pressure polymerization of urethane dimethacrylate. Dent Mater 31 (2015), 406–412.
[11] Tassin, M., Bonte, E., Loison-Robert, L.S., Nassif, A., Berbar, T., Le Goff, S., et al. Effects of high-temperature-pressure polymerized resin-infiltrated ceramic networks on oral stem cells. PLoS One, 11, 2016, e0155450.
[12] Gupta, S.K., Saxena, P., Pant, V.A., Pant, A.B., Release and toxicity of dental resin composite. Toxicol Int 19 (2012), 225–234.
[13] Lin-Gibson, S., Sung, L., Forster, A.M., Hu, H., Cheng, Y., Lin, N.J., Effects of filler type and content on mechanical properties of photopolymerizable composites measured across two-dimensional combinatorial arrays. Acta Biomater 5 (2009), 2084–2094.
[14] Neunzehn, J., Luttenberg, B., Wiesmann, H.P., Investigation of biomaterials by human epithelial gingiva cells: an in vitro study. Head Face Med, 8, 2012, 35.
[15] Okabe, E., Ishihara, Y., Kikuchi, T., Izawa, A., Kobayashi, S., Goto, H., et al. Adhesion properties of human oral epithelial-derived cells to zirconia. Clin Implant Dent Relat Res, 2015.
[16] Kimura, Y., Matsuzaka, K., Yoshinari, M., Inoue, T., Initial attachment of human oral keratinocytes cultured on zirconia or titanium. Dent Mater J 31 (2012), 346–353.
[17] Nothdurft, F.P., Fontana, D., Ruppenthal, S., May, A., Aktas, C., Mehraein, Y., et al. Differential behavior of fibroblasts and epithelial cells on structured implant abutment materials: a comparison of materials and surface topographies. Clin Implant Dent Relat Res 17 (2015), 1237–1249.
[18] Brunot-Gohin, C., Duval, J.L., Azogui, E.E., Jannetta, R., Pezron, I., Laurent-Maquin, D., et al. Soft tissue adhesion of polished versus glazed lithium disilicate ceramic for dental applications. Dent Mater 29 (2013), e205–e212.
[19] Forster, A., Ungvari, K., Gyorgyey, A., Kukovecz, A., Turzo, K., Nagy, K., Human epithelial tissue culture study on restorative materials. J Dent 42 (2014), 7–14.
[20] Pabst, A.M., Walter, C., Grassmann, L., Weyhrauch, M., Brullmann, D.D., Ziebart, T., et al. Influence of CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase release of human gingival fibroblasts and oral keratinocytes. Clin Oral Investig 18 (2014), 1111–1118.
[21] Grenade, C., De Pauw-Gillet, M.-C., Gailly, P., Vanheusden, A., Mainjot, A., Biocompatibility of polymer-infiltrated-ceramic-network (PICN) materials with human gingival fibroblasts (HGFs). Dent Mater 32 (2016), 1152–1164.
[22] Grenade, C., Moniotte, N., Rompen, E., Vanheusden, A., Mainjot, A., De Pauw-Gillet, M.-C., A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials. Cytotechnology 68 (2016), 2437–2448.
[23] Pendegrass, C.J., Lancashire, H.T., Fontaine, C., Chan, G., Hosseini, P., Blunn, G.W., Intraosseous transcutaneous amputation prostheses versus dental implants: a comparison between keratinocyte and gingival epithelial cell adhesion in vitro. Eur Cells Mater 29 (2015), 237–249.
[24] Pabst, A.M., Walter, C., Bell, A., Weyhrauch, M., Schmidtmann, I., Scheller, H., et al. Influence of CAD/CAM zirconia for implant-abutment manufacturing on gingival fibroblasts and oral keratinocytes. Clin Oral Investig 20 (2016), 1101–1108.
[25] Carre, A., Lacarriere, V., Mittal, K.L., (eds.) Contact Angle, Wettability and Adhesion, 5, 2008, VSP/Brill, Leiden, 253–267.
[26] Guelcher, S.A., Hollinger, J.O., An introduction to biomaterials. 2005.
[27] Linkevicius, T., Vaitelis, J., The effect of zirconia or titanium as abutment material on soft peri-implant tissues: a systematic review and meta-analysis. Clin Oral Implants Res 26 (2015), 139–147.
[28] Chen, H., Cohen, D.M., Choudhury, D.M., Kioka, N., Craig, S.W., Spatial distribution and functional significance of activated vinculin in living cells. J Cell Biol 169 (2005), 459–470.
[29] Shiraiwa, M., Goto, T., Yoshinari, M., Koyano, K., Tanaka, T., A study of the initial attachment and subsequent behavior of rat oral epithelial cells cultured on titanium. J Periodontol 73 (2002), 852–860.
[30] Nothdurft, F.P., Fontana, D., Ruppenthal, S., May, A., Aktas, C., Mehraein, Y., et al. Differential behavior of fibroblasts and epithelial cells on structured implant abutment materials: a comparison of materials and surface topographies. Clin Implant Dent Relat Res 17 (2015), 1237–1249.
[31] Zheng, M., Yang, Y., Liu, X.Q., Liu, M.Y., Zhang, X.F., Wang, X., et al. Enhanced biological behavior of in vitro human gingival fibroblasts on cold plasma-treated zirconia. PLoS One, 10, 2015, e0140278.
[32] An, N., Rausch-fan, X., Wieland, M., Matejka, M., Andrukhov, O., Schedle, A., Initial attachment, subsequent cell proliferation/viability and gene expression of epithelial cells related to attachment and wound healing in response to different titanium surfaces. Dent Mater 28 (2012), 1207–1214.
[33] Gómez-Florit, M., Ramis, J.M., Xing, R., Taxt-Lamolle, S., Haugen, H.J., Lyngstadaas, S.P., et al. Differential response of human gingival fibroblasts to titanium- and titanium-zirconium-modified surfaces. J Periodontal Res 49 (2014), 425–436.
[34] Klinge, B., Meyle, J., Working, G., Soft-tissue integration of implants. Consensus report of Working Group 2. Clin Oral Implants Res 17:Suppl. 2 (2006), 93–96.
[35] Kim, S.H., Ha, H.J., Ko, Y.K., Yoon, S.J., Rhee, J.M., Kim, M.S., et al. Correlation of proliferation, morphology and biological responses of fibroblasts on LDPE with different surface wettability. J Biomater Sci Polym Ed 18 (2007), 609–622.
[36] Lee, J.H., Khang, G., Lee, J.W., Lee, H.B., Interaction of different types of cells on polymer surfaces with wettability gradient. J Colloid Interface Sci 205 (1998), 323–330.
[37] Uo, M., Sjogren, G., Sundh, A., Watari, F., Bergman, M., Lerner, U., Cytotoxicity and bonding property of dental ceramics. Dent Mater 19 (2003), 487–492.
[38] Brackett, M.G., Lockwood, P.E., Messer, R.L., Lewis, J.B., Bouillaguet, S., Wataha, J.C., In vitro cytotoxic response to lithium disilicate dental ceramics. Dent Mater 24 (2008), 450–456.
[39] Messer, R.L., Lockwood, P.E., Wataha, J.C., Lewis, J.B., Norris, S., Bouillaguet, S., In vitro cytotoxicity of traditional versus contemporary dental ceramics. J Prosthet Dent 90 (2003), 452–458.
[40] Krifka, S., Spagnuolo, G., Schmalz, G., Schweikl, H., A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers. Biomaterials 34 (2013), 4555–4563.