Poster (Scientific congresses and symposiums)
Viral glycoprotein gp150 promotes sexual transmission of Murid Herpesvirus-4
Zeippen, Caroline; Javaux, Justine; Xiao, Xue et al.
2016Congrès Annuel de la Société Française d'Immunologie 2016
 

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Keywords :
Murid herpesvirus 4; viral transmission; glycoprotein
Abstract :
[en] Gammaherpesviruses are important pathogens in human and veterinary medicine. During co-evolution with their hosts, they developed many strategies allowing them to shed infectious particles in presence of immune response. Understanding these strategies is likely to be important to control infection. Interestingly, we recently observed that Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus infecting laboratory mice, could be sexually transmitted between mice. This model offers therefore the opportunity to understand the mechanisms underlying natural transmission. Some of these mechanisms could rely on the glycoprotein 150 (gp150), which could limit virus neutralization and promote the release of infectious particles from cells. In this study, we tested therefore the importance of gp150 in the context of MuHV-4 sexual transmission. Briefly, female mice were infected with WT or gp150- strains expressing luciferase. They were imaged with an in vivo imaging system to follow infection. When lytic replication was observed in the genital tract, infected females were mated with naïve males to compare the capacity of transmission of the two strains. Our results show that, while the gp150- strain has no deficit in reaching and replicating in the female genital tract, it displays a major deficit of sexual transmission in comparison with WT virions. Interestingly, this deficit appears to reflect a deficit of virions release from vaginal epithelial cells. Altogether, our results show that, while gp150 is not required for efficient dissemination and maintenance of MuHV-4 within its host, it is essential for efficient transmission, by promoting the releasing of infectious particles from the mucosal cells.
Research center :
laboratoire d'immunologie-vaccinologie, FMV, ULiège
Disciplines :
Microbiology
Author, co-author :
Zeippen, Caroline ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Javaux, Justine ;  Université de Liège > Immunologie et vaccinologie
Xiao, Xue ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Farnir, Frédéric  ;  Université de Liège > Département des productions animales (DPA) > Biostatistiques et bioinformatique appliquées aux sc. vétér.
Vanderplasschen, Alain ;  Université de Liège > Immunologie et vaccinologie
Stevenson, Philip;  university of Queensland and royal children's hospital > Queensland children's medical research institute and australian infectious disease research centre > sir albert sakzewski virus research centre
Gillet, Laurent  ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Language :
English
Title :
Viral glycoprotein gp150 promotes sexual transmission of Murid Herpesvirus-4
Publication date :
28 November 2016
Number of pages :
2*1m
Event name :
Congrès Annuel de la Société Française d'Immunologie 2016
Event organizer :
Société Française d'Immunologie
Event place :
Paris, France
Event date :
du 28 au 30 novembre 2016
Audience :
International
Name of the research project :
Importance des mécanismes d'évasion de la réponse immune humorale lors de la transmission des gammaherpèsvirus
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 22 January 2017

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