[en] We have identified the lef-1 genes from two multiple nucleopolyhedroviruses that infect
natural populations of Choristoneura fumiferana. The lef-I genes in both viruses are directly upstream and in the opposite orientation of their respective ecdysteroid UDP-glucosyltransferase (egt) genes. This gene organization pattern is similar to that found in the genomes of AcMNPV and of OpMNPV. As well, the coding regions and putative protein sequences share a high degree of similarity. Alignment of the predicted amino acid sequences of all known baculovirus lef-1 genes suggests that the LEF-1 proteins have a relatively high degree of conservation, particularly at four identified and distinct domains. Moreover, LEF-I proteins bear clear similarity to some eukaryotic primases, predominately at three of the four domains where certain amino acids are absolutely conserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Barrett, John W.
Lauzon, Hilary A.M.
Mercuri, Paola ; Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Krell, Peter J.
Sohi, S.S.
Arif, Basil M.
Language :
English
Title :
The Putative LEF-1 Proteins From Two Distinct Choristoneura fumiferana Multiple Nucleopolyhedroviruses Share Domain Homology to Eukaryotic Primases
Publication date :
1996
Journal title :
Virus Genes
ISSN :
0920-8569
eISSN :
1572-994X
Publisher :
Kluwer Academic Publishers, Boston, United States - Massachusetts
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