Article (Scientific journals)
Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV).
Garcia, Jose; Munro, Eann S.; Mira Monte, Milena et al.
2010In Fish and Shellfish Immunology, 29 (2), p. 293-7
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Keywords :
Animals; Antiviral Agents/isolation & purification/pharmacology; Birnaviridae Infections/veterinary/virology; Cell Line; Female; Fish Diseases/virology; Infectious pancreatic necrosis virus/drug effects; Salmo salar; Vitellogenins/isolation & purification/pharmacology
Abstract :
[en] Vitellogenin is a phosphoglycoprotein which represents the main precursor of the egg yolk in teleost fish. This reproductive protein was also demonstrated to play an important role in innate immunity by acting as a pattern recognition molecule capable of binding to bacteria, fungi and enhancing macrophage phagocytosis. The presented results demonstrate that, egg homogenate, ovarian fluid and serum of mature female Atlantic salmon have high neutralising ability for infectious pancreatic necrosis virus (IPNV). Vitellogenin from mature female Atlantic salmon serum, purified by immuno-affinity on a column matrix coated with monoclonal anti-Atlantic salmon vitellogenin antibody, was able to neutralise between 9.1 x 10(4) and 3.09 x 10(5) TCID(50) IPNV mg(-1) of protein. To the author's knowledge, this is the first time that the neutralising activity of vitellogenin on a teleost virus has been demonstrated. The results may explain why IPNV is difficult to detect by culture methods in ovarian fluid and egg homogenates from carrier mature females and suggest a possible means of vertical transmission via the egg.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Garcia, Jose
Munro, Eann S.
Mira Monte, Milena ;  Université de Liège > Immunologie et vaccinologie
Fourrier, Mickael ;  Université de Liège > Immunologie et vaccinologie
Whitelaw, Jason
Smail, David A.
Ellis, Anthony E.
Language :
English
Title :
Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV).
Publication date :
2010
Journal title :
Fish and Shellfish Immunology
ISSN :
1050-4648
eISSN :
1095-9947
Publisher :
Elsevier, United States
Volume :
29
Issue :
2
Pages :
293-7
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Crown Copyright 2010. Published by Elsevier Ltd. All rights reserved.
Available on ORBi :
since 16 January 2017

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