A simple LC-MS method for the determination of iohexol and iothalamate in serum, using ioversol as an internal standard.

Nyssen, Laurent; DELANAYE, Pierre; Le Goff, Caroline; PEETERS, Stéphanie; Cavalier, Etienne

doi:10.1016/j.cca.2016.10.021

Article (Scientific journals)

A simple LC-MS method for the determination of iohexol and iothalamate in serum, using ioversol as an internal standard.

Nyssen, Laurent; DELANAYE, Pierre; Le Goff, Caroline et al.

2016 • In Clinica Chimica Acta, 463, p. 96-102

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/205119

DOI
10.1016/j.cca.2016.10.021

PubMed
27756544

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Keywords :

Glomerular filtration rate; Iohexol; Iothalamate; Ioversol; LC-MS; Nephrology

Abstract :

[en] BACKGROUND: Chronic kidney disease (CKD) is diagnosed and explored through the determination of the glomerular filtration rate (GFR). Our goal was to develop a simple LC-MS method for the determination in serum of 2 popular GFR markers, contrast agents iohexol and iothalamate, for routine use and comparison studies between the two markers. A similar contrast agent, ioversol, was used as an internal standard and the method underwent a rigorous validation protocol based on beta-expectation tolerance intervals. METHODS: We adapted the HPLC-UV method from Cavalier et al. to our LC-MS system. Data treatment for the validation was performed using Multiquant 3.0 (Sciex, Framingham, MA, USA) and e.noval 3.0 software (Arlenda, Liege, Belgium). RESULTS: According to the validation results our method will give accurate and reliable results for concentrations ranging from 6.8 to 250mug/ml for iohexol and 6.15mug/ml to 250mug/ml for iothalamate. In our practice these intervals are sufficient to determine both compounds in most patient samples. Samples with higher detected concentrations can always be diluted into range. CONCLUSION: With its internal standard and extensive validation, our method is now ready for routine and clinical research use.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

Nyssen, Laurent ; Université de Liège > Département de pharmacie > Chimie médicale

DELANAYE, Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Service de néphrologie

Le Goff, Caroline ; Université de Liège > Département de pharmacie > Chimie médicale

PEETERS, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique

Cavalier, Etienne ; Université de Liège > Département de pharmacie > Chimie médicale

Language :

English

Title :

A simple LC-MS method for the determination of iohexol and iothalamate in serum, using ioversol as an internal standard.

Publication date :

2016

Journal title :

Clinica Chimica Acta

ISSN :

0009-8981

eISSN :

1873-3492

Publisher :

Elsevier, Netherlands

Volume :

463

Pages :

96-102

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 10 January 2017

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