Standardized protocol to evaluate pollen polypeptides as bee food source

[en] Bees mainly rely on pollen for their protein resources. As these molecules are essential for numerous aspects of bee physiology like ovary development and larval growth, their quantification and determination are crucial to evaluate diet quality. However, the term "protein" has been used to mention crude protein, total amino acids, or protein sensu stricto (i.e.; polypeptides of molecular weight >10,000 Da). In addition to this ambiguity, current methods for protein quantification suffer from bias due to nonprotein nitrogen and protein-to-protein variations. A reliable and nondestructive method to quantify the pollen polypeptides is then essential to estimate bee food source. The present paper aims (a) to detail such a protocol, (b) to evaluate its efficiency, and (c) to confront its results to those returned by traditional methods of protein estimation. Our protocol clearly overrides some bias of previous methods and is highly reliable. Results show the high variability in content of pollen polypeptides and suggest that the main part of the proteinaceous nitrogen is from oligopeptides. They also highlight that hand-collected pollen is a better matrice than pollen loads to estimate the polypeptides of pollen as bee food source. © 2013 INRA, DIB and Springer-Verlag France.

Disciplines :

Biochimie, biophysique & biologie moléculaire
Entomologie & lutte antiravageur
Sciences de l’environnement & écologie

Auteur, co-auteur :

Vanderplanck, Maryse ; University of Mons > Research Institute for Biosciences > Laboratory of Zoology

Leroy, B.; Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium

Wathelet, Bernard ; Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium

Wattiez, R.; Research Institute of Biosciences, Department of Proteomic and Microbiology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium

Michez, D.; Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium

Langue du document :

Anglais

Titre :

Standardized protocol to evaluate pollen polypeptides as bee food source

Date de publication/diffusion :

2014

Titre du périodique :

Apidologie

ISSN :

0044-8435

eISSN :

1297-9678

Maison d'édition :

Springer, Berlin, Allemagne

Volume/Tome :

Fascicule/Saison :

Pagination :

192-204

Peer reviewed :

Peer reviewed vérifié par ORBi

Disponible sur ORBi :

depuis le 10 janvier 2017

Statistiques

Nombre de vues

130 (dont 0 ULiège)

Nombre de téléchargements

1 (dont 0 ULiège)

Voir plus de statistiques

citations Scopus^®

citations Scopus^®
sans auto-citations

OpenCitations

citations OpenAlex

Bibliographie

Alaux, C.; Ducloz, F.; Crauser, D.; Le Conte, Y. (2010) Diet effects on honeybee immunocompetence. Biol. Lett. 6, 562-565
Alm, J.; Ohnmeiss, T.E.; Lanza, J.; Vriesenga, L. (1990) Preference of cabbage white butterflies and honey bees for nectar that contains amino acids. Oecologia 84, 53-57
Bremner, J.M.; Mulvaney, C.S. (1982) Nitrogen-total. In: Page, A.L.; Miller, R.H.; Keeney, D.R. (eds.) Methods of soil analysis. Part 2. Chemical and Microbiological Properties, pp. 595-624. American Society of Agronomy, Madison
Brodschneider, R.; Crailsheim, K. (2010) Nutrition and health in honey bees. Apidologie 41, 278-294
Buchmann, S.L. (1986) Vibratile pollination in Solanum and Lycopersicon: a look at pollen chemistry. In: D'Arcy, W.G. (ed.) Solanaceae II: Biology and Systematics, pp. 237-252. Columbia University Press, New York
Campos, M.G.; Cunha, A.; Markham, K.R. (1997) Bees-pollen. Composition, properties, and applications. In: Mizrahi, A.; Lensky, Y. (eds.) Bee Products: Properties, Applications, and Apitherapy, pp. 93-100. Plenum, New York
Clark, C.J.; Lintas, C. (1992) Chemical composition of pollen from kiwifruit vines. N. Z. J. Crop Hortic. 20, 337-344
Cohen, A.C. (2004) Insect Diet: Science and Technology. CRC, Boca Raton
Csonka, F.A.; Bernton, H.S.; Jones, D.B. (1925) Proteins of timothy and orchard grass pollen and their reaction to vernal hay fever. Proc. Soc. Exp. Biol. Med. 23, 14-16
de Brito Sanchez, M.G. (2011) Taste perception in honey bees. Chem. Senses 36(8), 675-692
De Groot, A.P. (1953) Protein and amino acid requirements of the honey bee (Apis mellifera). Physiol. Comp. Ecologia 3, 197-285
de Sá-Otero, M.D.P.; Armesto-Baztan, S.; Díaz-Losada, E. (2009) Analysis of protein content in pollen loads produced in north-west Spain. Grana 48, 290-296
Detzel, A.; Wink, M. (1993) Attraction, deterrence or intoxication of bees (Apis mellifera) by plant allelochemicals. Chemoecology 4, 8-18
Dobson, H.E.M.; Danielson, E.M.; Van Wese, I.D. (1999) Pollen odor chemicals as modulators of 534 bumble bee foraging on Rosa rugosa Thunb. (Rosaceae). Plant. Species Biol. 14, 153-166
Feuillet, D.; Odoux, J.F.; Mateescu, C.; Aupinel, P.; Lamy, H.; Moreau, N.; Roucher, L.; Souché, T. (2008) Evolution floristique et physico-chimique des pollens récoltés au cours de l'année 2006 sur le site de l'INRA du Magneraud. Bull. Tech. Apic. 35, 20-26
Forcone, A.; Aloisi, P.V.; Ruppel, S.; Munoz, M. (2011) Botanical composition and protein content of pollen collected by Apis mellifera L. in the north-west of Santa Cruz (Argentinean Patagonia). Grana 50, 30-39
Hanley, M.E.; Franco, M.; Pichon, S.; Darvill, B.; Goulson, D. (2008) Breeding system, pollinator choice and variation in pollen quality in British herbaceous plants. Funct. Ecol. 22, 592-598
Hartfelder, K, Gentile Bitondi, M.M.; Brent, C.; Guidugli-Lazzarini, K.R.; Simoes, Z.L.P.; Stabentheiner, A.; Donato Tanaka, E.; Wang, Y. (2013) Standard methods for physiology and biochemistry research in Apis mellifera. J. Apic. Res. 52(1): http://dx.doi.org/10.3896/IBRA.1.52.1.06
Herbert, E.W.; Shimanuki, H.; Caron, D. (1977) Optimum protein levels required by honey bees (Hymenoptera, Apidae) to initiate and maintain brood rearing. Apidologie 8, 141-146
Höcherl, N.; Siede, R.; Illies, I.; Gätschenberger, H.; Tautz, J. (2012) Evaluation of the nutritive value of maize for honey bees. J. Insect Physiol. 58, 278-285
Huang, T.; Jander, G.; de Vos, M. (2011) Non-protein amino acids in plant defense against insect herbivores: representative cases and opportunities for further functional analysis. Phytochemistry 72(13), 1531-1537
Hurd, P.D.; LaBerge, W.E.; Linsley, E.G. (1980) Principal sunflower bees of North America with emphasis on the southwestern United States (Hymenoptera: Apoidea). Smithson. Contrib. Zool. 310, 1-158
Kitaoka, T.K.; Nieh, J.C. (2009) Bumble bee pollen foraging regulation: role of pollen quality, storage levels, and odor. Behav. Ecol. Sociobiol. 63, 501-510
Knight, A.H.; Crooke, W.M.; Shepherd, H. (1972) Chemical composition of pollen with particular reference to cation exchange capacity and uronic acid content. J. Sci. Food Agric. 23, 263-274
Knox, D.A.; Shimanuki, H.; Herbert, E.W. (1971) Diet and longevity of adult honey bees. J. Econ. Entomol. 1415-1416
Kruger, N.J. (2002) The Bradford method for protein quantitation. In: Walker, J.M. (ed.) The Protein Protocols Handbook, 2nd edn, pp. 15-22. Humana, New Jersey
Lee, K.P.; Cory, J.S.; Wilson, K.; Raubenheimer, D.; Simpson, S.J. (2006) Flexible diet choice offsets protein costs of pathogen resistance in a caterpillar. Proc. R. Soc. B 273, 823-829
Lee, K.P.; Simpson, S.J.; Wilson, K. (2008) Dietary protein-quality influences melanization and immune function in an insect. Funct. Ecol. 22, 1052-1061
Leonhardt, S.D.; Blüthgen, N. (2012) The same, but different: pollen foraging in honeybee and bumblebee colonies. Apidologie 43, 449-464
Mayer, C.; Adler, L.S.; Armbruster, L.; Dafni, A.; Eardley, C.D.; et al. (2011) Pollination ecology in the 21st century: key questions for future research. J. Poll. Ecol. 3, 8-23
Michener, C.D. (2007) The Bees of the World. The Johns Hopkins University Press, Baltimore
Moore, J.C.; DeVries, J.W.; Lipp, M.; Griffiths, J.C.; Abernethy, D.R. (2010) Total protein methods and their potential utility to reduce the risk of food protein adulteration. Compr. Rev. Food Sci. F. 9, 330-357
Nicolson, S.W. (2011) Bee food: the chemistry and nutritional value of nectar, pollen and mixtures of the two. Afr. Zool. 46(2), 197-204
Nicolson, S.W.; Human, H. (2013) Chemical composition of the 'low quality' pollen of sunflower (Helianthus annuus, Asteraceae). Apidologie 44, 144-152
Odoux, J.-F.; Feuillet, D.; Aupinel, P.; Loublier, Y.; Taseï, J.-N.; Mateescu, C. (2012) Territorial biodiversity and consequences on physico-chemical characteristics of pollen collected by honey bee colonies. Apidologie 43, 561-575
Pernal, S.F.; Currie, R.W. (2002) Discrimination and preferences for pollen-based cues by foraging honeybees, Apis mellifera L. Anim. Behav. 63(2), 369-390
Quezada-Euan, J.J.G.; Lopez-Velasco, A.; Perez-Balam, J.; Moo-Valle, H.; Velazquez-Madrazo, A.; et al. (2011) Body size differs in workers produced across time and is associated with variation in the quantity and composition of larval food in Nannotrigona perilampoides (Hymenoptera, Meliponini). Insect. Soc. 58, 31-38
Robertson, A.W.; Mountjoy, C.; Faulkner, B.; Roberts, M.; Macnair, M. (1999) Bumblebee selection of Mimulus guttatus flowers: the effects of pollen quality and reward depletion. Ecology 80, 2594-2606
Roulston, T.H.; Cane, J.H. (2000) Pollen nutritional content and digestibility for animals. Plant Syst. Evol. 222, 187-209
Roulston, T.H.; Cane, J.H. (2002) The effect of pollen protein concentration on body size in the sweat bee Lasioglossum zephyrum (Hymenoptera: Apiformes). Evol. Ecol. 16, 49-65
Roulston, T.H.; Cane, J.H.; Buchmann, S.L. (2000) What governs protein content of pollen: pollinator preferences, pollen-pistil interaction, or phylogeny? Ecol. Monogr. 70, 617-643
Schmidt, J.O.; Hanna, A. (2006) Chemical nature of phagostimulants in pollen attractive to honeybees. J. Insect Behav. 19, 521-532
Sedivy, C.; Muller, A.; Dorn, S. (2011) Closely related pollen generalist bees differ in their ability to develop on the same pollen diet: evidence for physiological adaptations to digest pollen. Funct. Ecol. 25, 718-725
Somerville, D.C.; Nicol, H.I. (2006) Crude protein and amino acid composition of honey bee-collected pollen pellets from south-east Australia and a note on laboratory disparity. Aust. J. Exp. Agric. 46, 141-149
Stace, P. (1996) Protein content and amino acid profiles of honey bee-collected pollens. Bees 'n trees Consultants, Queensland
Standifer, L.N. (1967) A comparison of the protein quality of pollens for growth-stimulation of the hypopharyngeal glands and longevity of honey bees, Apis mellifera L. (Hymenoptera: Apidae). Insect Soc 14, 415-425
Standifer, L.N.; McCaughey, W.F.; Dixon, S.E.; Gilliam, M.; Loper, G.M. (1980) Biochemistry and microbiology of pollen collected by honey bees (Apis mellifera L.) from almond, Prunus dulcis. II. Protein, amino acids and enzymes. Apidologie 11, 163-171
Tasei, J.N.; Aupinel, P. (2008) Nutritive value of 15 single pollens and pollen mixes tested on larvae produced by bumblebee workers (Bombus terrestris, Hymenoptera: Apidae). Apidologie 39, 397-409
Vanderplanck, M.; Michez, D.; Vancraenenbroeck, S.; Lognay, G. (2011) Micro-quantitative method for analysis of sterol levels in honeybees and their pollen loads. Anal. Lett. 44, 1807-1820
Wang, W.; Vignani, R.; Scali, M.; Cresti, M. (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27, 2782-2786
Weiner, C.N.; Hilpert, A.; Werner, M.; Linsenmair, K.E.; Bluthgen, N. (2010) Pollen amino acids and flower specialisation in solitary bees. Apidologie 41, 476-487
Whitehead, A.T.; Larsen, J. (1976) Electrophysiological responses of galeal contact chemoreceptors to selected sugars and electrolytes. J. Insect Physiol. 22, 1609-1616