Article (Scientific journals)
Standardized protocol to evaluate pollen polypeptides as bee food source
Vanderplanck, Maryse; Leroy, B.; Wathelet, Bernard et al.
2014In Apidologie, 45 (2), p. 192-204
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Keywords :
BCA assay
Abstract :
[en] Bees mainly rely on pollen for their protein resources. As these molecules are essential for numerous aspects of bee physiology like ovary development and larval growth, their quantification and determination are crucial to evaluate diet quality. However, the term "protein" has been used to mention crude protein, total amino acids, or protein sensu stricto (i.e.; polypeptides of molecular weight >10,000 Da). In addition to this ambiguity, current methods for protein quantification suffer from bias due to nonprotein nitrogen and protein-to-protein variations. A reliable and nondestructive method to quantify the pollen polypeptides is then essential to estimate bee food source. The present paper aims (a) to detail such a protocol, (b) to evaluate its efficiency, and (c) to confront its results to those returned by traditional methods of protein estimation. Our protocol clearly overrides some bias of previous methods and is highly reliable. Results show the high variability in content of pollen polypeptides and suggest that the main part of the proteinaceous nitrogen is from oligopeptides. They also highlight that hand-collected pollen is a better matrice than pollen loads to estimate the polypeptides of pollen as bee food source. © 2013 INRA, DIB and Springer-Verlag France.
Disciplines :
Biochemistry, biophysics & molecular biology
Entomology & pest control
Environmental sciences & ecology
Author, co-author :
Vanderplanck, Maryse ;  University of Mons > Research Institute for Biosciences > Laboratory of Zoology
Leroy, B.;  Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium
Wathelet, Bernard ;  Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium
Wattiez, R.;  Research Institute of Biosciences, Department of Proteomic and Microbiology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium
Michez, D.;  Research Institute of Biosciences, Laboratory of Zoology, University of Mons UMONS, Place du parc 20, 7000 Mons, Belgium
Language :
English
Title :
Standardized protocol to evaluate pollen polypeptides as bee food source
Publication date :
2014
Journal title :
Apidologie
ISSN :
0044-8435
eISSN :
1297-9678
Publisher :
Springer, Berlin, Germany
Volume :
45
Issue :
2
Pages :
192-204
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 January 2017

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