Reference : Hepatoprotective effects of crude rhubarbe extract extend to the modulation of gut mi...
Scientific congresses and symposiums : Unpublished conference/Abstract
Life sciences : Veterinary medicine & animal health
Life sciences : Food science
http://hdl.handle.net/2268/203790
Hepatoprotective effects of crude rhubarbe extract extend to the modulation of gut microbiota in a murine acute alcohol-induced steato-hepatitis model
English
Taminiau, Bernard mailto [Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires >]
Neyrinck, A.M. []
Nezer, Carine []
Etxeberria, U. []
Burteau, Sophie []
Delzenne, N. []
Daube, Georges mailto [Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires >]
17-Oct-2014
No
National
FARAH Day
17 octobre 2014
Liège
Belgique
[en] Binge drinking is emerging and alarming social and health problem, leading to alcoholic liver disease (ALD). It is characterized by hepatic steatosis coupled with inflammation. A way to limit the evolution of ALD would be the use of bioactive compounds to reduce fat accumulation and inflammation. A specific crude rhubarb extract has been tested in a murine model of acute alcohol-induced steato-hepatitis. 18 male C57BL/6J mice has been fed with a standard diet, enriched with (OH-EV1 group) or without the rhubarb extract (CT and OH groups) for 17 days. Groups OH and OH-EV1 were then submitted to binge drinking and euthanazed. Physiological parameters have been measured. Moreover, bacterial ceacal contents were analyzed by pyrosequencing and 16S rDNA v1-V3 targeted metagenomic analysis.
Inflammatory markers levels in liver and total hepatic cholesterol level show that the standardized extract limits inflammation and lipid accumulation. Moreover, metagenomic analysis revealed the composition of major bacterial populations in caecum. Several populations were statistically influenced by the rhubarb enriched diet compared normal diet as shown after binge drinking. Among these populations, few were strongly correlated with the levels of inflammatory markers in the liver. We targeted 3 populations for further analysis : two Alistipes populations and Parabacteroides goldsteinii. The Alistipes belong to yet undescribed species. Based upon the only nucleic trace from pyrosequecing, 3 quantitative RT-PCR tests were created to target these populations in gut samples. Quantitative determination of these populations confirmed the statistical correlation with inflammatory markers and results from metagenomic study.
Researchers ; Professionals
http://hdl.handle.net/2268/203790

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