Reference : Liquid chromatography separation of the chiral prodrug eslicarbazepine acetate and it...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/202947
Liquid chromatography separation of the chiral prodrug eslicarbazepine acetate and its main metabolites in polar organic mode. Application to their analysis after in vitro metabolism.
English
Servais, Anne-Catherine mailto [Université de Liège > Département de pharmacie > Analyse des médicaments >]
Janicot, Bertrand [> >]
Takam, Arnold [> >]
Crommen, Jacques mailto [Université de Liège > Département de pharmacie > Département de pharmacie >]
Fillet, Marianne mailto [Université de Liège > Département de pharmacie > Analyse des médicaments >]
2016
Journal of Chromatography. A
1467
306-311
Yes (verified by ORBi)
International
0021-9673
1873-3778
Netherlands
[en] Chiral stationary phase ; Eslicarbazepine acetate ; In vitro metabolites ; Liquid chromatography
[en] A LC method using a chiral stationary phase (CSP) with cellulose tris(3-chloro-4-methylphenylcarbamate) as chiral selector in polar organic mode (POM) was developed for the separation of the biopharmaceutic classification system (BCS) class II chiral prodrug eslicarbazepine acetate (ESL) and its main metabolites, namely eslicarbazepine, its optical antipode, (R)-licarbazepine, and the achiral oxcarbazepine (OXC). The percentage of methanol (MeOH) in the mobile phase containing acetonitrile (ACN) as the main solvent was found to significantly influence analyte retention and resolution. A reversal of elution order of OXC and (R)-licarbazepine was observed, depending on the MeOH percentage in the mobile phase. The optimized mobile phase consisted of ACN/MeOH/acetic acid/diethylamine (95/5/0.2/0.07; v/v/v/v). The potential of this chemo- and enantioselective LC method combined with solid-phase extraction (SPE) was then evaluated for in vitro metabolism studies using ESL as a model case. Only eslicarbazepine could be detected after incubation of ESL in human liver microsome systems.
Centre Interdisciplinaire de Recherche sur le Médicament - CIRM
http://hdl.handle.net/2268/202947
10.1016/j.chroma.2016.07.022
Copyright (c) 2016 Elsevier B.V. All rights reserved.

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