Identification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with MHC class I molecules

Vanneste, Yves; Ntodou-Thomé, Agnès; Vandersmissen, Eric; Charlet-Renard, Jeanne de Chantal; Franchimont, Denis; Martens, Henri; Lhiaubet, Anne-Marie; Schimpff, Rose-Marie; Rostène, William; Geenen, Vincent

doi:10.1016/S0165-5728(97)00052-0

Article (Scientific journals)

Identification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with MHC class I molecules

Vanneste, Yves; Ntodou-Thomé, Agnès; Vandersmissen, Eric et al.

1997 • In Journal of Neuroimmunology, 76, p. 161-166

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/20202

DOI
10.1016/S0165-5728(97)00052-0

PubMed
9184646

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Abstract :

[en] This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain ± 5 ng immunoreactive (ir) NT/106 cells, of which 5% is associated with plasma cell membranes. HPLC analysis of NT-ir present in human TEC showed a major peak of NT-ir corresponding to NT1–13. NT-ir was not detected in the supernatant of human TEC cultures. Using an affinity column prepared with a anti-MHC class I monoclonal antibody, NT-ir-related peptides were retained on the column and eluted together with MHC class I-related proteins. According to the elution time on HPLC of these peptides, they correspond to intact NT1–13, as well as to smaller fragments of NT1–13.

Disciplines :

Endocrinology, metabolism & nutrition
Immunology & infectious disease

Author, co-author :

Vanneste, Yves

Ntodou-Thomé, Agnès

Vandersmissen, Eric

Charlet-Renard, Jeanne de Chantal ; Université de Liège - ULiège > Endocrinologie clinique

Franchimont, Denis

Martens, Henri ; Université de Liège - ULiège > Centre d'immunologie

Lhiaubet, Anne-Marie

Schimpff, Rose-Marie

Rostène, William

Geenen, Vincent ; Université de Liège - ULiège > Centre d'immunologie - Embryologie

Language :

English

Title :

Identification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with MHC class I molecules

Publication date :

1997

Journal title :

Journal of Neuroimmunology

ISSN :

0165-5728

eISSN :

1872-8421

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

Pages :

161-166

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 18 September 2009

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