Reference : Exploring potentialities of RanbP2-type Zinc Fingers in RNA-binding proteins design
Scientific congresses and symposiums : Poster
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/201845
Exploring potentialities of RanbP2-type Zinc Fingers in RNA-binding proteins design
English
De Franco, Simona mailto [Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines >]
Vandenameele, Julie mailto [Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines >]
Galleni, Moreno mailto [Université de Liège > Département des sciences de la vie > Macromolécules biologiques >]
Vandevenne, Marylène mailto [Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines >]
29-Jun-2016
A0
No
International
RNA 2016- The RNA Society of Japan 18th Annual Meeting and the 21st Annual Meeting of the RNA Society
du 28 juin 2016 au 2 juillet 2016
Kyoto
Japan
[en] RNA-binding ; zinc finger ; specificity
[en] Transcriptomes consist of several classes of RNAs, whose roles are central to innumerable biological processes as well as diseases. These observations justify the increasing interest in the engineering of functionalized RNA-binding proteins (RBPs) to specifically manipulate RNA function. In this context, the RanBP2-type Zinc Finger (ZF) domain emerged as a suitable scaffold for single-stranded RBPs design. The present study aimed to identify the sequence-specificity of several naturally occurring RanBP2-type ZFs by in vitro selection and use the natural variation in their substrate to create arrays of ZF domains displaying customized specificity. A parallel kinetic and thermodynamic characterization of ssRNA-ZFs interaction was performed by Isothermal Titration Calorimetry (ITC) and BioLayer Interferometry (BLI), respectively. Although our data showed that only little variations in the recognized sequence could be observed for the studied ZFs, they contribute to elucidate the molecular basis for the RanBP2-type ZF-ssRNA interaction.
Centre d'Ingénierie des Protéines - CIP
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Modular assembly of RanBP2-type Zinc figers to target single-stranded RNA
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/201845

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