RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis

[en] Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation, however their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatio-temporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Orman-Ligeza, Beata ; Université de Liège > Département des sciences de la vie > Physiologie végétale

Parizot, Boris

de Rycke, Riet

Fernandez, Ana

Ellie Himschoot, Ellie

Van Breusegem, Frank

Bennett, Malcolm

Périlleux, Claire ; Université de Liège > Département des sciences de la vie > Physiologie végétale

Beeckman, Tom

Draye, Xavier

Language :

English

Title :

RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis

Publication date :

2016

Journal title :

Development

ISSN :

0950-1991

eISSN :

1477-9129

Publisher :

Company of Biologists, Cambridge, United Kingdom

Special issue title :

Plant Development

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 14 September 2016

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