Article (Scientific journals)
The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.
Bolander, J.; Ji, W.; Geris, Liesbet et al.
2016In European Cells and Materials, 31, p. 11-25
Peer reviewed
 

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Abstract :
[en] When combining osteogenic progenitor cells such as human periosteum derived cells (hPDCs) with osteoconductive biomaterials like calcium phosphate (CaP)-scaffolds, in vivo bone formation can be achieved. This process is dependent on the early activation of Bone morphogenetic protein (BMP)-signalling. However, the bone forming process is slow and routinely only a limited amount of bone and bone marrow is formed. Therefore, we hypothesised that a robust clinically relevant outcome could be achieved by adding more physiological levels of potent BMP-ligands to these cell- and CaP-based constructs. For this, hPDCs were characterised for their responsiveness to BMP-ligands upon in vitro 2D stimulation. BMP-2, -4, -6 and -9 robustly induced osteochondrogenic differentiation. Subsequently, these ligands were coated onto clinically approved CaP-scaffolds, BioOss(R) and CopiOs(R), followed by hPDC-seeding. Protein lysates and conditioned media were investigated for activation of BMP signalling pathways. Upon in vivo implantation, the most abundant bone formation was found in BMP-2 and BMP-6-coated scaffolds. Implanted cells actively contributed to the newly formed bone. Remnants of cartilage could be observed in BMP-coated CopiOs(R)-constructs. Computational analysis displayed that the type of BMP-ligand as well as the CaP-scaffold affects skeletal tissue formation, observed in a qualitative as well as quantitative manner. Furthermore, the in vitro mechanism appears to predict the in vivo outcome. This study presents further evidence for the potential of BMP-technology in the development of clinically relevant cell-based constructs for bone regenerative strategies.
Disciplines :
Engineering, computing & technology: Multidisciplinary, general & others
Author, co-author :
Bolander, J.
Ji, W.
Geris, Liesbet  ;  Université de Liège > Département d'aérospatiale et mécanique > Génie biomécanique
Bloemen, V.
Chai, Y. C.
Schrooten, J.
Luyten, F. P.
Language :
English
Title :
The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.
Publication date :
2016
Journal title :
European Cells and Materials
eISSN :
1473-2262
Publisher :
Swiss Society for Biomaterials, Switzerland
Volume :
31
Pages :
11-25
Peer reviewed :
Peer reviewed
European Projects :
FP7 - 279100 - BRIDGE - Biomimetic process design for tissue regeneration: from bench to bedside via in silico modelling
Funders :
CE - Commission Européenne [BE]
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since 16 July 2016

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