[en] We report on a multi-sensor system for the parallel in-situ monitoring of cell nutrients, metabolites, cell density and pH in biotechnological processes. The fabrication process of the sensor chip, based on a CMOS process flow, is suitable for integration into standard processes and mass production of the system. The integration of enzyme sensors for wide concentration ranges, an impedance-based sensor and a pH sensor on one silicon chip for in-situ applications in bioreactors, fabricated in a CMOS-based process flow, is demonstrated for the first time to our best knowledge. Measurements of glucose and lactate concentrations in a wide range are shown with linear ranges up to 600 mMand 900 mM, respectively. Cell density is determined via conductivity changes of a cell suspension and measurements are performed up to 15 g/l dry cell weight. The pH sensor is based on C-V measurements at an electrolyte-insulator semiconductor
structure and able to measure pHin a range from pH3 to pH12. All sensors are successfully employed in the monitoring of cultures of Saccharomyces cerevisiae or Lactobacillus acidophilus.
Disciplines :
Electrical & electronics engineering
Author, co-author :
Mross, Stefan
Zimmermann, Tom
Winkin, N
Kraft, Michael ; Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes microélectroniques intégrés
Vogt, Holger
Language :
English
Title :
Integrated multi-sensor system for parallel in-situ monitoring of cell nutrients, metabolites, cell density and pH in biotechnological processes
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