[en] A recent study showed that silymarin, a standardized extract of S. marianum might be used in the prevention of equine laminitis. We investigated the effects of quercetin and some compounds found in silymarin (silybin, taxifolin and dehydrosilybin) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by stimulated equine neutrophils (PMNs) and on MPO activity. All compounds (tested between 100 nm and 100 mum) inhibited superoxide anion production by stimulated PMNs in a dose-dependent manner. Dehydrosilybin and quercetin inhibited superoxide production and MPO release from 10 mum. Classical MPO assay showed quercetin as the most potent inhibitor, followed by taxifolin, dehydrosilybin and silybin. SIEFED MPO assay highlighting the binding of tested compounds to MPO showed that only quercetin and taxifolin maintained an efficient inhibition above 90% at 10 mum. Altogether, our results showed a strong inhibition of PMN activation by planar compounds such as quercetin and dehydrosilybin and a strong inhibition of MPO activity by the smallest molecules, quercetin and taxifolin. In conclusion, the compounds from silymarin may be useful for modulating the oxidative response of PMNs, involved in the pathogenesis of laminitis, but further in vivo studies are needed.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Zholobenko, A.; Department of Medical Chemistry & Bio-chemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechRepublic
Mouithys-Mickalad, Ange ; Université de Liège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Modriansky, M.; Department of Medical Chemistry & Bio-chemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechRepublic
Serteyn, Didier ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Franck, Thierry ; Université de Liège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Language :
English
Title :
Polyphenols from Silybum marianum inhibit in vitro the oxidant response of equine neutrophils and myeloperoxidase activity.
Publication date :
2016
Journal title :
Journal of Veterinary Pharmacology and Therapeutics
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