Article (Scientific journals)
Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp
Kausel, Gudrun; Vera, María Inés; San Martin, Rody et al.
1999In Journal of Cellular Biochemistry, 75 (4), p. 598 - 609
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Keywords :
factor Pit-1; eurythermal ectotherms; carp
Abstract :
[en] A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat. J. Cell. Biochem. 75:598-609, 1999. © 1999 Wiley-Liss, Inc.
Research Center/Unit :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kausel, Gudrun;  Universidad Austral de Chile
Vera, María Inés;  Universidad Nacional Andrés Bello
San Martin, Rody;  Universidad Austral de Chile
Figueroa, Jaime E.;  Universidad Austral de Chile
Molina, Alfredo
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Krauskopf, Manuel;  Universidad Nacional Andrés Bello
Language :
English
Title :
Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp
Publication date :
09 November 1999
Journal title :
Journal of Cellular Biochemistry
ISSN :
0730-2312
eISSN :
1097-4644
Publisher :
Wiley Liss, Inc.
Volume :
75
Issue :
4
Pages :
598 - 609
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 August 2009

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