[en] Kefiran is a water-soluble polysaccharide, which can be isolated from kefir grains biomass. Rheological behaviour and physico-chemical characteristics of kefiran solutions were influenced by the extraction parameters. Degradation of the polymer chain occurred at the highest temperature tested (100 °C). The intrinsic viscosity of kefiran solutions (0.1 g·ml-1) varied between 8.24 mPa at 100 °C to 19.32 mPa at 80 °C. Regarding rheological properties, kefiran solutions had characteristics of a Newtonian behaviour in diluted solutions and pseudoplastic at higher concentrations.
High-performance liquid chromatography analysis of monosaccharides revealed that kefiran is composed of glucose and galactose in a relative molar ratio of 0.94–1.1. Infrared spectra of kefiran suggested the structure of α- and β-configurations in pyranose-form carbohydrates, which indicated a purified structure of kefiran. The molecular weight of kefiran polymer was between 2.4 × 106 Da and 1.5 × 107 Da, the values of molecular weight depending on extraction conditions. This polysaccharide was found to have higher intrinsic viscosity and higher apparent viscosity in aqueous solutions, which brings a perspective for its use as thickening or gelling agent in food, or as a matrix in film-forming solutions.
Disciplines :
Food science
Author, co-author :
Pop Rodica, Carmen; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca > Department of Food Engineering
Salanţă, Liana; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca > Department of Food Science
Rotar, Ancuţa M.; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca > Department of Food Science
Semeniuc, Cristina A.; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca > Department of Food Engineering
Socaciu, Carmen; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca > Department of Food Science
Sindic, Marianne ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Laboratoire Qualité et sécurité des produits agro-aliment.
Language :
English
Title :
Influence of extraction conditions on characteristics of microbial polysaccharide kefiran isolated from kefir grains biomass
Publication date :
26 March 2016
Journal title :
Journal of Food and Nutrition Research
ISSN :
1336-8672
Publisher :
VÚP Food Research Institute NAFC, Bratislava, Slovakia
Ghasemlou, M., Khodaiyan, F., Jahanbin, K., Gharibzahedi, S.M.T., Taheri, S., Structural investigation and response surface optimisation for improvement of kefiran production yield from a lowcost culture medium (2012) Food Chemistry, 133, pp. 383-389
Goršek, A., Tramšek, M., Kefir grains production-An approach for volume optimization of twostage bioreactor system (2008) Biochemical Engineering Journal, 42, pp. 153-158
Garbers, I.M., Britz, T.J., Witthuhn, R.C., PCRbased denaturing gradient gel electrophoretictypification and identification of the microbial consortium present in kefir grains (2004) World Journal of Microbiology and Biotechnology, 20, pp. 687-693
Tramšek, M., Goršek, A., A novel approach for assuring and following inoculum activity during kefir grains growth studies-Application of dynamic pH profile (2007) Acta Chimica Slovenica, 54, pp. 693-699. , http://acta-arhiv.chem-soc.si/54/54-4-693.pdf, ISSN: 1580-3155
Werning, M.L., Notararigo, S., Nácher, M., Fernández De Palencia, P., Aznar, R., López, P., Biosynthesis, purification and biotechnological use of exopolysaccharides produced by lactic acid bacteria (2012) Food additive., , El-Samragy, Y. (Ed.): Rijeka: InTech, ISBN: 978-953-51-0067-6
Ruas-Madiedo, P., Hugenholtz, J., Zoon, P., An overview of the functionality of exopolysaccharides produced by lactic acid bacteria (2002) International Dairy Journal, 12, pp. 163-171
Văidean, V.L., Determinants of EU-28 healthcare expenditure (2015) Journal of Economics, Business and Management, 3, pp. 1126-1130
La Rivieére, J.W.M., Kooiman, P., Schmidt, K., Kefiran, a novel polysaccharide produced in the kefir grain by Lactobacillus brevis (1967) Archives of Microbiology, 59, pp. 269-278
Cheirsilp, B., Shimizu, H., Shioya, S., Enhanced kefiran production by mixed culture of Lactobacillus kefiranofaciens and Saccharomyces cerevisiae (2003) Journal of Biotechnology, 100, pp. 43-53
Rimada, P.S., Abraham, A.G., Polysaccharide production by kefir grains during whey fermentation (2001) Journal of Dairy Research, 68, pp. 653-661
Zajšek, K., Kolar, M., Goršek, A., Characterisation of the exopolysaccharide kefiran produced by lactic acid bacteria entrapped within natural kefir grains (2011) International Journal of Dairy Technology, 64, pp. 544-548
Kooiman, P., The chemical structure of kefiran, the water-soluble polysaccharide of the kefir grain (1968) Carbohydrate Research, 7, pp. 200-211
Mironescu, M., Microbial polysaccharides. Production, characterisation and properties (2003) Acta Universitatis Cibiniensis Series E: Food Technology, 7 (2), pp. 26-38. , http://saiapm.ulbsibiu.ro/rom/cercetare/ACTA_E/AUCFT2003II%2026_38.pdf, ISSN: 1221-4973
Rottava, L.G., Batesini, M.F., Silva, L.L., Lerin, L., de Oliveira, D., Padilha, F.F., Toniazzo, G., Treichel, H., Xanthan gum production and rheological behaviour using different strains of Xanthomonas sp (2009) Carbohydrate Polymers, 77, pp. 65-71
Jindal, N., Singh, D.P., Khattar, J.S., Optimization, characterization, and flow properties of exopolysaccharides produced by the cyanobacterium Lyngbya stagnina (2013) Journal of Basic Microbiology, 53, pp. 1-11
Ninane, V., Mukandayambaje, R., Berben, G., Identification of lactic acid bacteria within the consortium of a kefir grain by sequencing 16S rDNA variable regions (2007) Journal of AOAC International, 90, pp. 1111-1117. , ISSN: 1060-3271
Pop, C., Apostu, S., Salanţă, L., Rotar, A.M., Sindic, M., Mabon, N., Socaciu, C., Influence of different growth conditions on the kefir grains production, used in the kefiran synthesis (2014) Bulletin UASVM Food Science and Technology, 71, pp. 147-153
Piermaria, J.A., De La Canala, M.L., Abrahama, A.G., Gelling properties of kefiran, a food-grade polysaccharide obtained from kefir grain (2008) Food Hydrocolloids, 22, pp. 1520-1527
Pop, C., Apostu, S., Rotar, A.M., Semeniuc, C.A., Sindic, M., Mabon, N., FTIR spectroscopy characterization of a new biofilm made from kefiran, a polysaccharide obtained from kefir grains (2013) Journal of Agroalimentary Processes and Technologies, 19, pp. 157-159. , http://orbi.ulg.ac.be/bitstream/2268/180031/1/FTIR%20spectroscopic%20characterization%20of%20a%20new%20biofilm%20obtained%20from%20kefiran.pdf, ISSN: 2069-0053
Gösta, B., (1995) Dairy processing handbook., , Lund: Tetra Pak Processing Systems AB
Leyva, A., Quintana, A., Sanchez, M., Rodriguez, E.N., Cremata, J., Sanchez, J.C., Rapid and sensitive anthrone sulfuric acid assay in microplate format to quantify carbohydrate in biopharmaceutical products: Method development and validation (2008) Biologicals, 36, pp. 134-141
Garna, H., Mabon, N., Wathelet, B., Paquot, M., New method for a two-step hydrolysis and chromatographic analysis of pectin neutral sugar chains (2004) Journal of Agricultural and Food Chemistry, 52, pp. 4652-4659
Moreno, J., Vargas, M.A., Madiedo, J.M., Muñoz, J., Rivas, J., Guerreo, M.G., Chemical and rheological properties of an extracellular polysaccharide produced by the cyanobacterium Anabaena sp. ATCC 333047 (2000) Biotechnology and Bioengineering, 67, pp. 283-290
Piermaria, J., Pinotti, A., García, M.A., Abraham, A.G., Films based on kefiran, an exopolysaccharide obtained from kefir grain: Development and characterization (2009) Food Hydrocolloids, 23, pp. 684-690
Kalashnikov, V.N., Shear-rate dependent viscosity of dilute polymer solutions (1994) Journal of Rheology, 38, p. 1385
Rao, M.A., (2007) Rheology of fluid and semisolid foods principles and applications., , 2nd edition. New York: Springer Science, ISBN-13: 978-1461492290
Badel, S., Bernardi, T., Michaud, P., Research review paper-New perspectives for Lactobacilli exopolysaccharides (2011) Biotechnology Advances, 29, pp. 54-66
Liu, J.R., Chen, M.J., Lin, C.W., Characterization of polysaccharide and volatile compounds produced by kefir grains grown in soymilk (2002) Journal of Food Science, 67, pp. 104-108
Cerqueira, M.A., Souza, B.W.S., Teixeira, J.A., Vicente, A.A., Effect of glycerol and corn oil on physicochemical properties of polysaccharide films-A comparative study (2012) Food Hydrocolloids, 27, pp. 175-184
Piermaria, J., Bosch, A., Pinotti, A., Yantorno, O., Garcia, M.A., Abraham, A.G., Kefiran films plasticized with sugars and polyols: Water vapor barrier and mechanical properties in relation to their microstructure analyzed by ATR/FT-IR spectroscopy (2011) Food Hydrocolloids, 25, pp. 1261-1269
Villetti, M.A., Crespo, J.S., Soldi, M.S., Pires, A.T.N., Borsali, R., Soldi, V., Thermal degradation of natural polymers (2002) Journal of Thermal Analysis and Calorimetry, 67, pp. 295-303
Zhao, G., Kan, J., Li, Z., Chen, Z., Structural features and immunological activity of a polysaccharide from Dioscorea opposite Thunb roots (2005) Carbohydrate Polymers, 61, pp. 125-131
Kačuráková, M., Capek, P., Sasinkova, V., Wellner, N., Ebringerova, A., FT-IR study of plant cell wall model compounds: Pectic polysaccharides and hemicelluloses (2000) Carbohydrate Polymers, 43, pp. 195-203
Ahmed, Z., Wang, Y., Anjum, N., Ahmad, A., Khan, S.T., Characterization of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir-Part II (2013) Food Hydrocolloids, 30, pp. 343-350
Wang, M., Bi, J., Modification of characteristics of kefiran by changing the carbon source of Lactobacillus kefiranofaciens (2008) Journal of the Science of Food and Agriculture, 88, pp. 763-769
Villay, A., Lakkis De Filippis, F., Picton, L., Le Cerf, D., Vial, C., Michaud, P., Comparison of polysaccharide degradations by dynamic highpressure homogenization (2012) Food Hydrocolloids, 27, pp. 278-286
Kim, J.H., Kim, M.R., Lee, J.H., Lee, J.W., Kim, S.K., Production of high molecular weight pullulan by Aureobasidium pullulans using glucosamine (2000) Biotechnology Letters, 22, pp. 987-990
Santos, M., Rodrigues, A., Teixeira, J.A., Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512 (2005) Biochemical Engineering Journal, 25, pp. 1-6
Faria, S., Petkowicz, C.L.O., de Morais, S.A.L., Terrones, M.G.H., de Resende, M.M., de Franca, F.P., Cardoso, V.L., Characterization of xanthan gum produced from sugar cane broth (2011) Carbohydrate Polymers, 86, pp. 469-476
Amin, K.A.M., Panhuis, M., Polyelectrolyte complex materials from chitosan and gellan gum (2011) Carbohydrate Polymers, 86, pp. 352-358
