Real-time Recordings of Migrating Cortical Neurons from GFP and Cre Recombinase Expressing Mice.

[en] The cerebral cortex is one of the most intricate regions of the brain that requires elaborate cell migration patterns for its development. Experimental observations show that projection neurons migrate radially within the cortical wall, whereas interneurons migrate along multiple tangential paths to reach the developing cortex. Tight regulation of the cell migration processes ensures proper positioning and functional integration of neurons to specific cerebral cortical circuits. Disruption of neuronal migration often leads to cortical dysfunction and/or malformation associated with neurological disorders. Unveiling the molecular control of neuron migration is thus fundamental to understanding the physiological or pathological development of the cerebral cortex. In this unit, protocols allowing detailed analysis of patterns of migration of both interneurons and projection neurons under different experimental conditions (i.e., loss or gain of function) are presented.

Disciplines :

Neurology

Author, co-author :

Tielens, Sylvia ; Université de Liège > GIGA - Neurosciences

Godin, Juliette D.

Nguyen, Laurent ; Université de Liège > GIGA - Neurosciences

Language :

English

Title :

Real-time Recordings of Migrating Cortical Neurons from GFP and Cre Recombinase Expressing Mice.

Publication date :

2016

Journal title :

Current Protocols in Neuroscience

ISSN :

1934-8584

eISSN :

1934-8576

Publisher :

Wiley-Liss Inc, United States

Volume :

Pages :

3.29.1-23

Commentary :

Available on ORBi :

since 24 May 2016

Statistics

Number of views

81 (11 by ULiège)

Number of downloads

2 (2 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Bellion, A., Baudoin, J.P., Alvarez, C., Bornens, M., and Metin, C. 2005. Nucleokinesis in tangentially migrating neurons comprises two alternating phases: Forward migration of the Golgi/centrosome associated with centrosome splitting and myosin contraction at the rear. J. Neurosci. 25: 5691-5699. doi: 10.1523/JNEUROSCI.1030-05.2005.
Evsyukova, I., Plestant, C., and Anton, E.S. 2013. Integrative mechanisms of oriented neuronal migration in the developing brain. Annu. Rev. Cell Dev. Biol. 29: 299-353. doi: 10.1146/annurev-cellbio-101512-122400.
Godin, J.D., Thomas, N., Laguesse, S., Malinouskaya, L., Close, P., Malaise, O., Purnelle, A., Raineteau, O., Campbell, K., Fero, M., Moonen, G., Malgrange, B., Chariot, A., Metin, C., Besson, A., and Nguyen, L. 2012. p27(Kip1) is a microtubule-associated protein that promotes microtubule polymerization during neuronmigration. Dev. Cell 23: 729-744. doi: 10.1016/j.devcel.2012.08.006.
Gupta, A., Tsai, L.H., and Wynshaw-Boris, A. 2002. Life is a journey: Agenetic look at neocortical development. Nat. Rev. Genet. 3: 342-355. doi: 10.1038/nrg799.
Marin, O., Valiente, M., Ge, X., and Tsai, L.H. 2010. Guiding neuronal cell migrations. Cold Spring Harb. Perspect. Biol. 2: a001834. doi: 10.1101/cshperspect.a001834.
Metin, C., Vallee, R.B., Rakic, P., and Bhide, P.G. 2008. Modes and mishaps of neuronal migration in the mammalian brain. J. Neurosci. 28: 11746-11752. doi: 10.1523/JNEUROSCI.3860-08.2008.
Nadarajah, B., Brunstrom, J.E., Grutzendler, J., Wong, R.O., and Pearlman, A.L. 2001. Two modes of radial migration in early development of the cerebral cortex. Nat. Neurosci. 4: 143-150. doi: 10.1038/83967.
Noctor, S.C., Martinez-Cerdeno, V., Ivic, L., and Kriegstein, A.R. 2004. Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases. Nat. Neurosci. 7: 136-144. doi: 10.1038/nn1172.
Pacary, E. and Guillemot, F. 2014. In utero electroporation to study mouse brain development. Methods Mol. Biol. 1082: 285-293. doi: 10.1007/978-1-62703-655-9_19.
Rakic, P. 1972. Mode of cell migration to the superficial layers of fetal monkey neocortex. J. Comp. Neurol. 145: 61-83. doi: 10.1002/cne.901450105.
Riedl, J., Crevenna, A.H., Kessenbrock, K., Yu, J.H., Neukirchen, D., Bista, M., Bradke, F., Jenne, D., Holak, T.A., Werb, Z., Sixt, M., and Wedlich-Soldner, R. 2008. Lifeact: A versatile marker to visualize F-actin. Nat. Methods 5: 605-607. doi: 10.1038/nmeth.1220.
Saito, T. 2015. In Utero Electroporation of the Mouse Embryo. In Electroporation Methods in Neuroscience, Vol. 102, pp. 1-20. Springer, New York.
Stenman, J., Toresson, H., and Campbell, K. 2003. Identification of two distinct progenitor populations in the lateral ganglionic eminence: Implications for striatal and olfactory bulb neurogenesis. J. Neurosci. 23: 167-174.
Stuhmer, T., Anderson, S.A., Ekker, M., andRubenstein, J.L. 2002. Ectopic expression of the Dlx genes induces glutamic acid decarboxylase and Dlx expression. Development 129: 245-252.
Valiente, M. and Marin, O. 2010. Neuronal migration mechanisms in development and disease. Curr. Opin. Neurobiol. 20: 68-78. doi: 10.1016/j.conb.2009.12.003.
van den Berghe, V., Stappers, E., Vandesande, B., Dimidschstein, J., Kroes, R., Francis, A., Conidi, A., Lesage, F., Dries, R., Cazzola, S., Berx, G., Kessaris, N., Vanderhaeghen, P., van Ijcken, W., Grosveld, F.G., Goossens, S., Haigh, J.J., Fishell, G., Goffinet, A., Aerts, S., Huylebroeck, D., and Seuntjens, E. 2013. Directed migration of cortical interneurons depends on the cell-autonomous action of Sip1. Neuron 77: 70-82. doi: 10.1016/j.neuron.2012.11.009.
Volvert, M.L., Prevot, P.P., Close, P., Laguesse, S., Pirotte, S., Hemphill, J., Rogister, F., Kruzy, N., Sacheli, R., Moonen, G., Deiters, A., Merkenschlager, M., Chariot, A., Malgrange, B., Godin, J.D., and Nguyen, L. 2014. MicroRNA targeting of CoREST controls polarization of migrating cortical neurons. Cell Rep. 7: 1168-1183. doi: 10.1016/j.celrep.2014.03.075.