SH2 domain containing inositol 5-phosphatases 1 and 2 in blood platelets: interaction and respective role in the control of phosphatidylinositol 3,4,5-trisphosphate levels
[en] Src homology domain 2-containing inositol 5-phosphatases 1 and 2 (SHIP1 and SHIP2) are capable of dephosphorylating the second messenger PtdIns(3,4,5) P3 (phosphatidylinositol 3,4,5-trisphosphate) and interacting with several signalling proteins. SHIP1 is essentially expressed in haematopoietic cells, whereas SHIP2, a closely related enzyme, is ubiquitous. In the present study, we show that SHIP1 and SHIP2 are expressed as functional PtdIns(3,4,5) P3 5-phosphatases in human blood platelets and are capable of interacting when these two lipid phosphatases are co-expressed, either naturally (platelets and A20 B lymphoma cells) or artificially (COS-7 cells). Using COS-7 cells transfected with deletion mutants of SHIP2, we demonstrate that the Src homology domain 2 of SHIP2 is the minimal and sufficient protein motif responsible for the interaction between the two phosphatases. These results prompted us to investigate the relative importance of SHIP1 and SHIP2 in the control of PtdIns(3,4,5) P3 levels in platelets using homozygous or heterozygous SHIP1- or SHIP2-deficient mice. Our results strongly suggest that SHIP1, rather than SHIP2, plays a major role in controlling PtdIns(3,4,5) P3 levels in response to thrombin or collagen activation of mouse blood platelets
Giuriato, S.; INSERM U563, Hôpital Purpan, 31059 Toulouse Cedex, France > Department of Oncogenesis and Signaling in Hematopoietic Cells, IFR30, Hˆopital Purpan, 31059 Toulouse Cedex, France
Pesesse, X.; Université Libre de Bruxelles - ULB > IRIBHM
Bodin, S.; INSERM U563, Hôpital Purpan, 31059 Toulouse Cedex, France > Department of Oncogenesis and Signaling in Hematopoietic Cells, IFR30
Sasaki, T.; Akita University School of Medicine, 1-1-1 Hondo, 010-8543, Akita, Japan, > The 21st Century Center of Excellence (COE) Program
Viala, C.; INSERM U563, Hôpital Purpan, 31059 Toulouse Cedex, France > Department of Oncogenesis and Signaling in Hematopoietic Cells, IFR30
Marion, E.; Université Libre de Bruxelles - ULB > Centre de Recherche en Biotechnologie, Interdisciplinary Research Institute (IRIBHM)
Penninger, J.; Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr Bohr Gasse 7, 1030 Vienna, Austria
Schurmans, Stéphane ; Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie métabolique vétérinaire
Erneux, C.; Université Libre de Bruxelles - ULB > IRIBHM
Payrastre, B.; INSERM U563, Hôpital Purpan, 31059 Toulouse Cedex, France > Department of Oncogenesis and Signaling in Hematopoietic Cells, IFR30
Language :
English
Title :
SH2 domain containing inositol 5-phosphatases 1 and 2 in blood platelets: interaction and respective role in the control of phosphatidylinositol 3,4,5-trisphosphate levels
Publication date :
2003
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
376
Pages :
199-207
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE] INSERM - Institut National de la Santé et de la Recherche Médicale [FR] CFB - Communauté française de Belgique [BE] Fondation ARC pour la Recherche sur le Cancer [FR]
Funding text :
This work was supported by the CEE BIOMED 2 contract no. PL962609, by grants from Action de Recherche Concertée (Communauté française de Belgique Wallonie, Bruxelles, Belgium), Fonds de la Recherche Scientifique Médicale, Association pour la Recherche sur le Cancer (Villejuif, France; contract no. 4794 and ARECA-Toulouse) and the INSERM–Communauté française de Belgique exchange contract.
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