Reference : Restricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts a...
Scientific journals : Article
Human health sciences : Oncology
Restricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts adjacent to human breast cancer cells
Bisson, C. [> > > >]
Blacher, Silvia mailto [Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Polette, M. [> > > >]
Blanc, J. F. [> > > >]
Kebers, F. [> > > >]
Desreux, Joëlle mailto [Centre Hospitalier Universitaire de Liège - CHU > > Sénologie >]
Tetu, B. [> > > >]
Rosenbaum, J. [> > > >]
Foidart, Jean-Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique >]
Birembaut, P. [> > > >]
Noël, Agnès mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
International Journal of Cancer = Journal International du Cancer
Wiley Liss, Inc.
Yes (verified by ORBi)
New York
[en] MTI-MMP ; myofibroblast ; cancer ; stroma ; alpha smooth muscle actin ; MMP-2
[en] The membrane type-1 matrix metalloproteinase (MT1-MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to activate other MMPs (MMP-2 and MMP-13) and to degrade extracellular matrix. Our study was undertaken to localize and identify the MT1-MMP expressing cells in human breast adenocarcinomas. A textural analysis of images obtained by immunohistochemistry and in situ hybridization showed precisely the co-expression of alpha smooth muscle actin (alphaSM actin) and MT1-MMP in myofibroblasts. MT1-MMP expression is confined to myofibroblasts in close contact with tumor cells. In sharp contrast, the expression of MMP-2 was more widely distributed in both alphaSM actin positive and negative cells close to and at distance from cancer cell clusters. Our in vitro observations are consistent with the higher level of MT1-MMP expression and of MMP-2 activation observed in alphaSM actin positive fibroblasts derived from breast tumors, as compared to normal breast fibroblasts. Collectively, these results implicate myofibroblasts as major producer of MT1-MMP in breast cancer and emphasize the importance of stromal-epithelial cell interactions in their progression.

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