Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

[en] Background: Ovarian tissue preservation is proposed to patients at risk of premature ovarian failure, but this procedure still needs to be optimized. To limit injury during ovarian tissue cryopreservation, anti-apoptotic drugs were added to the transport and freezing media of ovarian cortex tissue. Methods: Sheep ovaries were transported, prepared and frozen in solutions containing vehicle or anti-apoptotic drugs (Z-VAD-FMK, a pan-caspase inhibitor, or sphingosine-1-phosphate (S1P), a bioactive lipid). After the tissue was thawed, the ovarian cortex was cultured for 2 or 6 days. Follicular quantification and morphological and proliferation analyses were performed on histological sections. Results: After 2 days of culture, S1P improved the quality of primordial follicles; higher densities of morphologically normal and proliferative primordial follicles were found. Z-VAD-FMK displayed similar effects by preserving global primordial follicular density, but this effect was evident after 6 days of culture. This drug also improved cell proliferation after 2 and 6 days of culture. Conclusions: Our results showed that the addition of S1P or Z-VAD-FMK to the transport and freezing media prior to ovarian tissue cryopreservation improves primordial follicular quality and therefore improves global tissue survival. This should ultimately lead to improved fertility restoration after auto-transplantation.

Disciplines :

Reproductive medicine (gynecology, andrology, obstetrics)

Author, co-author :

HENRY, Laurie ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR

Fransolet, Maïté ; Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

LABIED, Soraya ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR

Blacher, Silvia ; Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

MASEREEL, Marie-Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR

Foidart, Jean-Michel ; Université de Liège > Département des sciences cliniques > Département des sciences cliniques

Noël, Agnès ; Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Nisolle, Michelle ; Université de Liège > Département des sciences cliniques > Gynécologie - Obstétrique

Munaut, Carine ; Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Language :

English

Title :

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

Publication date :

2016

Journal title :

Journal of Ovarian Research

eISSN :

1757-2215

Publisher :

BioMed Central, London, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 March 2016

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