[en] Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of various gene deletions (gC, gE, gI, gG) on the induction of cell-mediated immune responses against the virus. The highest BHV1 specific lymphoproliferative response was observed in the group of calves inoculated with the gC- mutant. However, in all groups of inoculated calves, limiting dilution analysis showed marked individual variability in the number of BHV1 specific T lymphocytes that were stimulated. The same animals were then challenged with wild-type BHV1. In these animals, limiting dilution analysis did not reveal gE, gI nor gG as a major T lymphocyte antigen. However, further analysis suggested the T cell antigenicity of gE in a low number of BHV1 hyperimmunized calves. Stimulation of MHC unrestricted cytotoxicity was also evaluated after inoculation with the various deletion mutants. Cytotoxicity in gC- inoculated calves was as high as in BHV1 inoculated calves. In conclusion, among the BHV1 deletion mutants that were tested, the gC- mutant stimulated the best cell-mediated immune responses.
Disciplines :
Veterinary medicine & animal health Microbiology
Author, co-author :
Denis, M.
Hanon, E.
Rijsewijk, F. A.
Kaashoek, M. J.
van Oirschot, J. T.
Thiry, Etienne ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
The Role of Glycoproteins gC, gE, gI and gG in the Induction of Cell-Mediated Immune Responses to Bovine Herpesvirus 1
Campos, M., Rossi, C.R., Cell-mediated cytotoxicity of bovine mononuclear cells to IBRV-infected cells: Dependence on Sephadex G-10 adherent cells (1985) Vet. Immunol. Immunopathol., 8, pp. 363-375
Campos, M., Bielefeldt Ohmann, H., Hutchings, D., Rapin, N., Babiuk, L.A., Lawman, M.J.P., Role of interferon-γ in inducing cytotoxicity of peripheral blood mononuclear leukocytes to bovine herpesvirus type 1 (BHV-1)-infected cells (1989) Cell. Immunol., 120, pp. 259-269
Campos, M., Griebel, P., Bielefeldt Ohmann, H., Babiuk, L.A., Cell-mediated cytotoxic responses in lungs following a primary bovine herpes virus type 1 infection (1992) Immunology, 75, pp. 47-52
Denis, M., Slaoui, M., Keil, G., Babiuk, L.A., Ernst, E., Pastoret, P.-P., Thiry, E., Identification of different target glycoproteins for bovine herpes virus type 1-specific cytotoxic T lymphocytes depending on the method of in vitro stimulation (1993) Immunology, 78, pp. 7-13
Denis, M., Kaashoek, M.J., Van Oirschot, J.T., Pastoret, P.-P., Thiry, E., Quantitative assessment of the specific CD4+ T lymphocyte response in bovine herpesvirus 1 immune cattle (1994) Vet. Immunol. Immunopathol., 42, pp. 275-286
Hutchings, D.L., Van Drunen Littel-Van Den Hurk, S., Babiuk, L.A., Lymphocyte proliferative responses to separated bovine herpesvirus 1 proteins in immune cattle (1990) J. Virol., 64, pp. 5114-5122
Kaashoek, M.J., Moerman, A., Madic, J., Rijsewijk, F.A.M., Quak, J., Gielkens, A.L.J., Van Oirschot, J.T., A conventionally attenuated glycoprotein E-negative strain of bovine herpesvirus type 1 is an efficacious and safe vaccine (1994) Vaccine, 12, pp. 439-444
Kaashoek, M.J., Rijsewijk, F.A.M., Paal, H., Ruuls, R., Gielkens, A.L.J., Van Oirschot, J.T., In vivo phenotypes and immunogenic properties of bovine herpesvirus 1 mutants with a deletion in the gC. gG. gI or gE gene (1994) Proc. III Congr. of the European Society for Veterinary Virology, , Interlaken, Switzerland
Leary, T.P., Splitter, G.A., Recombinant herpesviral proteins produced by cell-free translation provide a novel approach for the mapping of T lymphocyte epitopes (1990) J. Immunol., 145, pp. 718-723
Liang, X., Babiuk, L.A., Zamb, T.J., An in vivo study of a glycoprotein gIII-negative bovine herpesvirus 1 (BHV1) mutant expressing β-galactosidase: Evaluation of the role of gIII in virus infectivity and its use as a vector for mucosal immunization (1992) Virology, 189, pp. 629-639
Mettenleiter, Th.C., Pseudorabies (Aujeszky's disease) virus: State of the art, August 1993 (1994) Acta Vet. Hung., 42, pp. 153-177
Reddehase, M.J., Cox, J.H., Koszinowski, U.H., Frequency analysis of cytolytic T cell precursors (CTL-P) generated in vivo during lethal rabies infection of mice. 1. Distinction of CTL-P with different interleukin 2 sensitivity (1982) Eur. J. Immunol., 12, pp. 519-523
Rijsewijk, F.A.M., Magdalena, J., Moedt, J., Kaashoek, M.J., Maris-Velduis, M.A., Gielkens, A.L.J., Van Oirschot, J.T., Identification and functional analysis of glycoprotein E (gE) of bovine herpesvirus type 1 (1992) Proc. XVII Int. Herpesvirus Workshop, , Edinburgh
Rijsewijk, F.A.M., Kaashoek, M.J., Madic, J., Paal, H., Ruuls, R., Gielkens, A.L.J., Van Oirschot, J.T., Characterization of a DNA rearrangement found in the unique short region of the Za strain of bovine herpesvirus type 1 and the vaccine properties of this strain (1993) Proc. XVIII Int. Herpesvirus Workshop, , Pittsburgh, PA
Rouse, B.T., Babiuk, L.A., The direct antiviral cytotoxicity by bovine lymphocytes is not restricted by genetic incompatibility of lymphocytes and target cells (1977) J. Immunol., 118, pp. 618-624
Rutten, V.P.M.G., Wentink, G.H., De Jong, W.A.C., Van Exsel, A.C.A., Hensen, E.J., Determination of BHV1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays (1990) Vet. Immunol. Immunopathol., 25, pp. 259-267
Stegeman, J.A., Tielen, M.J.M., Kimman, T.G., Van Oirschot, J.T., Hunneman, W.A., Berndsen, F.W., Intensive regional vaccination with a gI-deleted vaccine markedly reduces pseudorabies virus infections (1994) Vaccine, 12, pp. 527-531