Effects of Sodium Naproxen on Differentiated Human Chondrocytes Cultivated in Clusters

[en] The effects of different pharmacological concentrations of the non-steroidal anti-inflammatory drug (NSAID) sodium naproxen (NAP) were tested on several metabolic functions of differentiated human chondrocytes cultivated in clusters and compared with the action of acetylsalicylic acid (ASA). DNA synthesis was significantly inhibited by ASA but not by NAP. Proteoglycan production was also markedly decreased by ASA, while synthesis of type II collagen was not modified. By contrast, NAP did not affect these chondroformative processes. Both NSAIDs were potent inhibitors of prostaglandin E2 production. These results indicate that in terms of the parameters tested NAP does not lead to deleterious effects on human articular chondrocytes cultured in vitro.

Disciplines :

Rheumatology

Author, co-author :

Bassleer, C.

Henrotin, Yves ; Université de Liège - ULiège > Unité de recherche sur l'os et le cartillage (U.R.O.C.)

Franchimont, P.

Language :

English

Title :

Effects of Sodium Naproxen on Differentiated Human Chondrocytes Cultivated in Clusters

Publication date :

March 1992

Journal title :

Clinical Rheumatology

ISSN :

0770-3198

eISSN :

1434-9949

Publisher :

Springer, Germany

Volume :

Issue :

Pages :

60-5

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 21 January 2016

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Bibliography

Lequesne M. (1981) Arthrose de la hanche et du genou. Critères de diagnostic. Indices de mesure de la douleur, de la fonction et du résultat thérapeutique. Epidemiology of Osteoarthritis , J.G., Peyron, Geigy, Basel; 198-210.
Palmoski M.J., Brandt K.D. (1979) Effect of calcipenia on proteoglycan metabolism and aggregation in normal articular cartilage in vitro. Biochem J 182:399-406.
Palmoski M.J., Brandt K.D. (1979) Effect of salicylate on proteoglycan metabolism in normal canine articular cartilage in vitro. Arthritis Rheum 22:746-754.
Palmoski M.J., Brandt K.D. (1980) Effects of some nonsteroidal antiinflammatory drugs on proteoglycan metabolism and organization in canine articular cartilage. Arthritis Rheum 23:1010-1020.
Palmoski M.J., Brandt K.D. (1982) Partial reversal of beta-D-xyloside of salicylate-induced inhibitor of glycosaminoglycan synthesis in articular cartilage. Arthritis Rheum 25:1084-1093.
Palmoski M.J., Brandt K.D. (1983) Relationship between matrix PG content and the effects of salicylate and indomethacin on articular cartilage. Arthritis Rheum 26:528-531.
Verbruggen G., Veys E., Malfait A.M., Cochez P., Schatteman L., Wieme N., Heynen G., Broddelez C. (1989) Proteoglycan metabolism in tissue cultured human articular cartilage. Influence of piroxicam. J Rheumatol 16:355-362.
Mitrovic D., McCall E., Dray F. (1982) The in vitro production of prostanoids by cultured bovine articular chondrocytes. Prostaglandins 23:17-28.
Mitrovic D., McCall E., Front D., Aprice F., Darmon N., Dray F. (1984) Anti-inflammatory drugs, prostanoids and proteoglycan production by cultured bovine articular chondrocytes. Prostaglandins 28:417-434.
Kirkpatrick C.J., Mohr W., Wildfeuer A., Haferkamp O. (1983) Influence of nonsteroidal anti-inflammatory agents on lapine articular chondrocytes growth in vitro. J Rheumatol 42:58-65.
Fontagne J., Loizeau M., Adolphe M., Lechat P. (1984) Effects of indomethacin on collagen biosynthesis by rabbit articular chondrocytes in monolayer culture. Int J Tissue React 6:233-241.
De Vries B.J., Van Den Berg W.B., Van de Putte L.B. (1985) Salicylate-induced depletion of endogenous inorganic sulfate. Arthritis Rheum 28:922-929.
Galera P., Hartmann D., Daireaux M., Mauviel A., Loyau G., Pujol J.P. (1989) Effet du naproxène sur la production de collagène par les fibroblastes dermiques, les synoviocytes et les chondrocytes articulaires stimulés par l'interleukine-1. Actualités en Physiopathologie et Pharmacologie Articulaires , A., Gaucher, J., Pourel, P., Netter, M., Kessler, Masson, Paris; 223-234.
Bassleer C., Henrotin Y., Bassleer R., Franchimont P. (1989) Effets de l'etodolac sur le métabolisme des chondrocytes humains cultivés en agrégats. Actualités en Physiopathologie et Pharmacologie Articulaires , A., Gaucher, J., Pourel, P., Netter, M., Kessler, Masson, Paris; 239-240.
Bassleer C., Henrotin Y., Franchimont P. (1990) In vitro assays of chondrocyte functions: the influence of drugs and hormones. Scand J Rheumatol 81:13-20.
Franchimont P., Bassleer C., Henrotin Y., Gysen P., Bassleer R. (1989) Effects of human and salmon calcitonin on human articular chondrocytes cultivated in clusters. J Clin Endocrin Metab 69:259-266.
Bassleer C., Gysen P., Foidart J.M., Bassleer R., Franchimont P. (1986) Human chondrocytes in tridimensional culture. In vitro 22:113-119.
Bassleer C., Gysen P., Bassleer R., Franchimont P. (1988) Effects of peptidic glycosaminoglycan complex on human chondrocytes cultivated in three dimensions. Biochem Pharmacol 37:1939-1945.
Levy G. (1979) Pharmacokinetics of salicylate in men. Drug Metab Rev 9:3-19.
Dougados M., Coste P., Amor B. (1985) Dosage du naproxène sodique dans le sang et dans le liquide synovial. J Intern Med 21-26.
Gysen P., Franchimont P. (1984) Radioimmunoassay of proteoglycans. J Immunoassay 5:221-243.
Oegema T.R., Hascall V.C., Dziewatkowski D.D. (1975) Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma. J Biol Chem 250:6151-6159.
Henrotin Y., Bassleer C., Nusgens B., Franchimont P. (1990) Radioimmunoassay for human type II collagen. J Immunoassay 11:555-578.
Herbage D., Bouillet J., Bernengo J.C. (1977) Biochemical and physiochemical characterization of pepsin-solubilized type II collagen from bovine articular cartilage. Biochem J 161:303-312.
Labarca C., Paigen K. (1980) A simple, rapid and sensitive DNA assay procedure. Anal Biochem 102:344-352.
Serteyn D., Deby-Dupont G., Pincemail J., Mottart E., Philipart C., Lamy P. (1988) Equine post-anaesthesic myositis: thromboxane, prostacyclin and prostaglandin E2 production. Vet Res Com 12:219-226.
Kuettner K.E., Pauli B.O., Gall G., Memoli V.A., Schenk R.K. (1982) Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics and morphology. J Cell Biol 93:743-750.
Kuettner K.E., Memoli V.A., Pauli B.U., Wrobel N.C., Thonar E., Daniel J.C. (1982) Synthesis of cartilage matrix by mammalian chondrocytes in vitro. II. Maintenance of collagen and proteoglycan phenotype. J Cell Biol 93:751-757.
Harmand M.F., Duphil R., Blanquet P. (1982) Proteoglycan synthesis in chondrocyte cultures from osteoarthritic and normal human articular cartilage. Biochem Biophys Acta 717:190-202.
Franchimont P., Bassleer C. (1989) New diagnostic tools and methodological approaches: an outlook to the future. Scand J Rheumatol 80:29-31.
Benya P.D., Shaffer J.D. (1982) Dedifferentiated chondrocytes reexpress the differentiated phenotype when cultured in agarose. Cell 20:215-224.
.
Burkhardt D., Ghosh P. (1987) Laboratory evaluation of antiarthritic drugs as potential chondroprotective agents. Semin Arthritis Rheum 17:3-34.
Lee K.H., Spencer M.R. (1969) Studies on the mechanics of action of salicylates. J Pharmacol Sci 58:464-472.
Hanks G.W. (1983) Non-prescription analgesics: Acetylsalicylic acid. Clin Ther 5:1-22.
Naprosyn (Naproxen) Clinical and Technical Review, Fleming, Syntex Laboratories Inc., Palo Alto, CA; 1984.
Hucker H.B., Stauffer S.C., White S.D., Rhodes R.E., Arisoa B.H., Umbenhauer E.R., Bower R.J., McMahon F.G. (1973) Physiologic disposition and metabolic fate of a new anti-inflammatory agent, cis-5-fluoro-2-methyl-1-methylsulfulin-benzylidenyl-3-acetate acid in the rat, dog, rhesus monkey and man. Drug Metab Dispos 2:721-736.
Rubin A., Warrick P., Woden R.L., Chernish S.M., Ridolfo A.S., Gruber C.M. (1972) Physiological disposition of fenoprofen. J Pharmacol Exp Ther 183:449-457.
Zini R., D'Athis P., Barre J., Tillement J.P. (1979) Binding of indomethacin to human serum albumin: its nondisplacement by various agents, influence of free fatty acids and the unexpected effect of indomethan on warfarin binding. Biochem Pharmacol 28:2661-2665.
Palmoski M.J., Brandt K.D. (1984) Effects of salicylate and indomethacin on GAG and PGE2 synthesis in intact canine knee cartilage in vivo. Arthritis Rheum 27:398-403.
Palmoski M.J., Brandt K.D. (1985) Proteoglycan depletion rather than fibrillation determines the effects of salicylate and indomethacin on osteoarthritic cartilage. Arthritis Rheum 28:548-553.
Muir H., Carney S.L., Hall L.G. (1988) Effects of tiaprofenic acid and other NSAIDs on proteglycan metabolism in articular cartilage explants. Drugs 35:15-23.
De Vries B.J., Van Den Berg W.B., Vitters E., Van De Putte L.B. (1988) Effects of NSAIDs on the metabolism of sulphated glycosaminoglycans in healthy and (post) arthritic murine articular cartilage. Drugs 35:24-32.
Fujii K., Tajiri K., Kajiwara T., Tanaka T., Murota K. (1989) Effects of NSAIDs on collagen and proteoglycan synthesis of cultured chondrocytes. J Rheumatol 16:19-28.
Vignon E., Richard M., Mathieu P., Conrozier T. (1990) Effets des antiinflammatoires non-stéroidiens sur la dégradation enzymatique du cartilage arthrosique. Rev Rhum 57:255-259.
.
.