Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase, and lignin changes in relation to vitrification of carnation tissues cultured in vitro
[en] Vitrification of carnation tissues by passage from solid to liquid medium was accompanied by decreased lignin content. The higher lignin level of normal tissues corresponded to a higher phenylalanine ammonia-lyase activity. It also paralleled a much higher activity of soluble and wall peroxidases towards syringaldazine. Kinetical analysis of soluble isoperoxidases indicated a two-step control of basic and acidic enzymes with a diminished activity of the latter in glassy plants. Compartment analysis of guaiacol peroxidases showed a different distribution in malformed tissues.
Kevers, Claire ; Université de Liège > Département des sciences de la vie > Département des sciences de la vie
Gaspar, Thomas; Université de Liège - ULiège
Language :
English
Title :
Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase, and lignin changes in relation to vitrification of carnation tissues cultured in vitro
Alibert G., Boudet A. (1979) La lignification chez le peuplier. I. Mise au point d'une méthode de dosage et d'analyse monomérique des lignines. Physiol. Vég 17:67-74.
Boyer N., Desbiez M.O., Hofinger M., Gaspar (1983) Effect of lithium on thigmomorpho-genesis in Bryonia dioica. Ethylene production and sensitivity. Plant Physiol 72:522-525.
Darimont E., Penel C., Auderset G., Greppin H., Gaspar (1977) Peroxydases de haut poids moléculaire identifiées à des peroxydases membranaires chez la Lentille. Arch, inter. Physiol. Biochim 85:497-507.
Darimont E., Schachhofer K., Gaspar TH. (1973) lsoperoxydases et hydroxyproline dans les parois cellulaires des racines de Lentille. Biochimica et Biophysica Acta (BBA) - Enzymology 461-466.
.
Earle E., Langhans R. (1975) Carnation propagation from shoot tips cultured in liquid medium. Hort Sci 10:608-610.
Fleuriet A., Deloire A. (1982) Aspects histochimiques et biochimiques de la cicatrisation des fruits de tomate blessés. Zeitschrift für Pflanzenphysiologie 259-268.
Gaspar TH., Penel C., Thorpe T.A., Greppin H. A Survey of their Biochemical and Physiological Roles in Higher Plants , Univ. de Genève-Centre de Botanique publ., Genève; 1982, 1970-1980.
Goldberg R., Catesson A.M., Czaninski Y. (1981) Histochemical and biochemical characteristics of peroxidases involved in lignification processes in poplar. Cell Walls , Quader; 81:251-260.
Goldberg R., Catesson A.M., Czaninski Y. (1983) Some properties of syringaldazine oxidase, a peroxidase specifically involved in the lignification processes. Zeitschrift für Pflanzenphysiologie 110:267-279.
Grand C., Ranjeva R. (1979) La lignification chez le peuplier. III. Variation du niveau d'activité d'enzymes impliquées dans la biosynthèse des monomères en fonction du degré de lignification et de la nature des tissus. Physiol. Vég 17:433-444.
Johnson D.B., Moore W.E., Zank L.C. (1961) The spectrophotometric determination of lignins in small wood samples. TAPPI (Technical Association of the Pulp and Pepper Industry) 44:793-798.
Kevers C., Coumans M., Coumans-Gilles M.F., Gaspar (1984) Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiologia Plantarum 61:69-74.
Letouze R. (1975) Croissance d'un bourgeon axillaire chez Salix babylonica L et activité phenylalanine ammoniac-lyase. Planta 123:155-162.
Letouze R., Daguin F. (1983) Manifestation spontanée et aléatoire d'une croissance anormale en culture in vitro. Recherche de marqueurs métaboliques. Rev. Can. Biol. Exp 42:23-28.
Paynot M., Melin D., Martin C. (1971) Existence d'un gradient d'activité phenylalanine ammoniac-lyase chez Nicotiana tabacum var xanthi n.c et Periploca graeca. L. C.R. Acad. Sei. Paris 273:749-751.
Penel C., Darimont E., Greppin H., Gaspar (1979) Rôle du calcium dans l'association des peroxydases à des membranes de racine de Lentille. C.R. Acad. Sei. Paris 289:529-533.
Penel C., Greppin H. (1979) Effect of calcium on subcellular distribution of peroxidases. Phyto-chemistry 18; 29-33.
Roberts L.W., Miller A.R. (1983) Is ethylene involved in xylem differentiation?. Vistas Plant Sei 6:1-24.
Spector T. (1978) Refinement of the Coomasie blue method of protein quantitation. Anal. Biochem 86:142-146.
Westcott R.J., Henshaw G.G. (1976) Phenolic synthesis and phenylalanine ammonia-lyase activity in suspension cultures of Acer pseudoplatanus L. Planta 131:67-72.
