MicroRNA-124 Regulates Cell Specification in the Cochlea through Modulation of Sfrp4/5.

[en] The organ of Corti, the auditory organ of the mammalian inner ear, contains sensory hair cells and supporting cells that arise from a common sensory progenitor. The molecular bases allowing the specification of these progenitors remain elusive. In the present study, by combining microarray analyses with conditional deletion of Dicer in the developing inner ear, we identified that miR-124 controls cell fate in the developing organ of Corti. By targeting secreted frizzled-related protein 4 (Sfrp4) and Sfrp5, two inhibitors of the Wnt pathway, we showed that miR-124 controls the β-catenin-dependent and also the PCP-related non-canonical Wnt pathways that contribute to HC differentiation and polarization in the organ of Corti. Thus, our work emphasizes the importance of miR-124 as an epigenetic safeguard that fine-tunes the expression of genes critical for cell patterning during cochlear differentiation.

Disciplines :

Otolaryngology
Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Huyghe, Aurelia ^✱

Van Den Ackerveken, Priscilla ^✱; Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine

SACHELI, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie clinique

Prevot, Pierre-Paul ; Université de Liège > GIGA - Neurosciences

Thelen, Nicolas ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Renauld, Justine ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Thiry, Marc ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Delacroix, Laurence ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Nguyen, Laurent ; Université de Liège > GIGA - Neurosciences

Malgrange, Brigitte ; Université de Liège > GIGA - Neurosciences

^✱ These authors have contributed equally to this work.

Language :

English

Title :

MicroRNA-124 Regulates Cell Specification in the Cochlea through Modulation of Sfrp4/5.

Publication date :

2015

Journal title :

Cell Reports

eISSN :

2211-1247

Publisher :

Elsevier

Volume :

Pages :

31-42

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 January 2016

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