First detection of CTX-M-28 in a Tunisian hospital from a cefotaxime-resistant Klebsiella pneumoniae strain.

Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins/chemistry/genetics; Cefotaxime/pharmacology; Cross Infection/epidemiology/microbiology; DNA, Bacterial/genetics; Drug Resistance, Multiple, Bacterial/genetics; Hospitals, Military; Humans; Intensive Care Units; Klebsiella Infections/epidemiology/microbiology; Klebsiella pneumoniae/classification/genetics/isolation & purification; Molecular Sequence Data; Mutation, Missense; R Factors/genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tunisia/epidemiology; beta-Lactam Resistance/genetics; beta-Lactamases/chemistry/genetics

Abstract :

[en] A cefotaxime-resistant Klebsiella pneumoniae ML4313 was obtained from a patient from intensive care unit of Military hospital in Tunisia. This strain was resistant to beta-lactams, aminoglycosides, quinolones and phenicols, and tetracyclines. It was identified as producer of extended-spectrum beta-lactamases (ESBL) by double-disk synergy test between amoxicillin-clavulanate and cefotaxime, ceftriaxone, ceftazidime and aztreonam. The ESBL was identified as CTX-M-28 by sequencing of PCR products and by isoelectric focusing. The ESBL resistance was transferred by a 50kb plasmid. CTX-M-28 is closely related to CTX-M-15. This is the first description of this enzyme in Tunisia.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Ben Achour, N.

Mercuri, Paola ; Université de Liège > Département des sciences de la vie > Macromolécules biologiques

Power, P.

Belhadj, C.

Ben Moussa, M.

Galleni, Moreno ; Université de Liège - ULiège

Belhadj, O.

Language :

English

Title :

First detection of CTX-M-28 in a Tunisian hospital from a cefotaxime-resistant Klebsiella pneumoniae strain.

Publication date :

2009

Journal title :

Pathologie Biologie

ISSN :

0369-8114

eISSN :

1768-3114

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

343-8

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 November 2015

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