[en] RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 A resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH5_5 subfamily members.
Adams, P D. et al. (2010). Acta Cryst. D66, 213-221
Aghajari, N., Feller, G., Gerday, C. & Haser, R. (1998) Protein Sci. 7, 564-572
Aspeborg, H., Coutinho, P. M., Wang, Y., Brumer, H. & Henrissat, B. (2012). BMC Evol. Biol. 12, 186
Berlemont, R., Delsaute, M., Pipers, D., D'Amico, S., Feller, G., Galleni, M. & Power, P. (2009). ISME J. 3, 1070-1081
Berlemont, R., Pipers, D., Delsaute, M., Angiono, F., Feller, G., Galleni, M. & Power, P. (2011). Rev. Argent. Microbiol. 43, 94-103
Bielecki, S., Krystynowicz, A., Turkiewicz, M. & Kalinowska, H. (2005). Bacterial Cellulose.Weinheim:Wiley-VCH. doi:10.1002/3527600035.bpol5003
Brzozowski, A. M., Lawson, D. M., Turkenburg, J. P., Bisgaard-Frantzen, H., Svendsen, A., Borchert, T. V., Dauter, Z., Wilson, K. S. & Davies, G. J. (2000). Biochemistry, 39, 9099-9107
Chen, V. B., Arendall, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S. & Richardson, D. C. (2010). Acta Cryst. D66, 12-21
Chen, C.-C., Guo, W.-J. & Isselbacher, K. J. (1987). Biochem. J. 247, 715-724
Correia, M. A., Mazumder, K., Brá s, J. L., Firbank, S. J., Zhu, Y., Lewis, R. J., York,W. S., Fontes, C. M. & Gilbert, H. J. (2011). J. Biol. Chem. 286, 22510- 22520
Cutfield, S. M., Davies, G. J., Murshudov, G., Anderson, B. F., Moody, P. C., Sullivan, P. A. & Cutfield, J. F. (1999). J. Mol. Biol. 294, 771-783
Davies, G. J. (1998). Biochem. Soc. Trans. 26, 167-173
Davies, G. J., Wilson, K. S. & Henrissat, B. (1997). Biochem. J. 321, 557-559
Domi'nguez, R., Souchon, H., Lascombe, M. & Alzari, P. M. (1996). J. Mol. Biol. 257, 1042-1051
Ducros, V., Czjzek, M., Belaich, A., Gaudin, C., Fierobe, H.-P., Belaich, J.-P., Davies, G. J. & Haser, R. (1995). Structure, 3, 939-949
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486-501
Harris, G. W., Jenkins, J. A., Connerton, I. & Pickersgill, R. W. (1996). Acta Cryst. D52, 393-401
Holm, L., Ka?a? ria? inen, S., Rosenstro?m, P. & Schenkel, A. (2008). Bioinformatics, 24, 2780-2781
Holm, L. & Rosenstro?m, P. (2010). Nucleic Acids Res. 38, W545-W549
Juers, D. H., Huber, R. E. & Matthews, B. W. (1999). Protein Sci. 8, 122-136
Kabsch, W. (2010). Acta Cryst. D66, 133-144
Kersters-Hilderson, H., Loontiens, F. G., Claeyssens, M. & De Bruyne, C. K. (1969). Eur. J. Biochem. 7, 434-441
Kim, H. W. & Ishikawa, K. (2011). Biochem. J. 437, 223-230
Krieger, E., Darden, T., Nabuurs, S. B., Finkelstein, A. & Vriend, G. (2004). Proteins, 57, 678-683
Laskowski, R. A. & Swindells, M. B. (2011). J. Chem. Inf. Model. 51, 2778- 2786
Linden, A., Mayans, O., Meyer-Klaucke, W., Antranikian, G. & Wilmanns, M. (2003). J. Biol. Chem. 278, 9875-9884
Lee, T. M., Farrow, M. F., Arnold, F. H. & Mayo, S. L. (2011). Protein Sci. 20, 1935-1940
Lo Leggio, L. & Larsen, S. (2002). FEBS Lett. 523, 103-108
Mazur, O. & Zimmer, J. (2011). J. Biol. Chem. 286, 17601-17606
Myrback, K. & Schilling, W. (1965). Enzymologia, 29, 306-314
Pell, G., Szabo, L., Charnock, S. J., Xie, H., Gloster, T. M., Davies, G. J. & Gilbert, H. J. (2004). J. Biol. Chem. 279, 11777-11788
Pettersen, E. F., Goddard, T. D., Huang, C. C., Couch, G. S., Greenblatt, D. M., Meng, E. C. & Ferrin, T. E. (2004). J. Comput. Chem. 25, 1605-1612
Pickersgill, R., Harris, G., Lo Leggio, L., Mayans, O. & Jenkins, J. (1998). Biochem. Soc. Trans. 26, 190-198
Robledo, M., Rivera, L., Jime'nez-Zurdo, J. I., Rivas, R., Dazzo, F., Vela'zquez, E., Marti'nez-Molina, E., Hirsch, A. M. & Mateos, P. F. (2012). Microb. Cell Fact. 11, 125.
Ro? mling, U. (2002). Res. Microbiol. 153, 205-212
Sabini, E., Schubert, H., Murshudov, G., Wilson, K. S., Siika-Aho, M. & Penttila?, M. (2000). Acta Cryst. D56, 3-13
Sakon, J., Adney, W. S., Himmel, M. E., Thomas, S. R. & Karplus, P. A. (1996). Biochemistry, 35, 10648-10660
Sidhu, G., Withers, S. G., Nguyen, N. T., McIntosh, L. P., Ziser, L. & Brayer, G. D. (1999). Biochemistry, 38, 5346-5354
Smith, N., Witham, S., Sarkar, S., Zhang, J., Li, L., Li, C. & Alexov, E. (2012). Bioinformatics, 28, 1655-1657
Vocadlo, D. J. & Davies, G. J. (2008). Curr. Opin. Chem. Biol. 12, 539-555
Wilkins, M. R., Gasteiger, E., Bairoch, A., Sanchez, J. C., Williams, K. L., Appel, R. D. & Hochstrasser, D. F. (1999). Methods Mol. Biol. 112, 531-552
Zheng, B., Yang,W., Zhao, X., Wang, Y., Lou, Z., Rao, Z. & Feng, Y. (2012). J. Biol. Chem. 287, 8336-8346.
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