[en] Human, bovine, and porcine insulins are small proteins with very closely related amino acid sequences, which makes their separation challenging. In this study, we took advantage of the high-resolution power of CE, and more particularly of micellar electrokinetic chromatography, to separate those biomolecules. Among several surfactants, perfluorooctanoic acid ammonium salt was selected. Then, using a design of experiments approach, the optimal BGE composition was found to consist of 50 mM ammonium acetate pH 9.0, 65 mM perfluorooctanoic acid ammonium salt, and 4% MeOH. The three insulins could be separated within 12 min with a satisfactory resolution. This method could be useful to detect possible counterfeit pharmaceutical formulations. Indeed, it would be easy to determine if human insulin was replaced by bovine or porcine insulin.
Research Center/Unit :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Lamalle, Caroline ; Université de Liège > Département de pharmacie > Analyse des médicaments
Roland, Diane ; Université de Liège - ULiège > 2e an. master sc. pharma., fin spéc. prat. offici.
Crommen, Jacques ; Université de Liège > Département de pharmacie > Département de pharmacie
Servais, Anne-Catherine ; Université de Liège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ; Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Separation of human, bovine, and porcine insulins, three very closely related proteins, by micellar electrokinetic chromatography.
