[en] We report on an electrochemical measurement setup comprising a glucose sensor and a CMOS potentiostat with a two-layer membrane as the first steps toward the development of an integrated in-situ sensor system for bioreactors. The potentiostat has a chip size of 2.1 mm× 2.5 mm and a linear current range from −220 nA to 240 nA with a linearity of R2 = 0.9995. For wide range measurements of glucose concentrations in cell culture media, electrodes functionalized with the
enzyme glucose oxidase were spin-coated with membranes made from polydimethylsiloxane (PDMS). A two-stage curing scheme of the PDMS was applied, and different membrane thicknesses
and curing times were evaluated. With these membranes, glucose concentrations up to 500 mM were measured with a linear measurement range up to 200 mM. The sensors were successfully
employed in the glucose monitoring of a culture of Saccharomyces cerevisiae to monitor the glucose consumption of the cells. For interference elimination, the cellulose acetate membranes were employed.
Disciplines :
Electrical & electronics engineering
Author, co-author :
Mross, Stefan
Fuerst, Peter
Pierrat, Sebastien
Vogt, Holger
Kraft, Michael ; Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes microélectroniques intégrés
Language :
English
Title :
Enzyme sensor with Polydimethylsiloxane membrane and CMOS potentiostat for wide-range glucose measurements.
Publication date :
2015
Journal title :
IEEE Sensors Journal
ISSN :
1530-437X
eISSN :
1558-1748
Publisher :
Institute of Electrical and Electronics Engineers, New York, United States - New York
M. Pohlscheidt, S. Charaniya, C. Bork, M. Jenzsch, T. L. Noetzel, and A. Luebbert, "Bioprocess and fermentation monitoring," in Upstream Industrial Biotechnology, vol. 2, M. C. Flickinger, Ed., 1st ed. Hoboken, NJ, USA: Wiley, 2013, ch. 5, sec. 69, pp. 1471-1491.
A. F. Europa, A. Gambhir, P.-C. Fu, and W.-S. Hu, "Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells," Biotechnol. Bioeng., vol. 67, no. 1, pp. 25-34, Jan. 2000.
L. Li et al., "Increasing the culture efficiency of hybridoma cells by the use of integrated metabolic control of glucose and glutamine at low levels," Biotechnol. Appl. Biochem., vol. 42, no. 1, pp. 73-80, Aug. 2005.
S. S. Farid, "Process economics of industrial monoclonal antibody manufacture," J. Chromatograph. B, vol. 848, no. 1, pp. 8-18, Mar. 2007.
S. Spichiger and U. E. Spichiger-Keller, "Process monitoring with disposable chemical sensors fit in the framework of process analysis technology (PAT) for innovative pharmaceutical development and quality assurance," CHIMIA Int. J. Chem., vol. 64, no. 11, pp. 803-807, 2010.
P. Hossler, S. F. Khattak, and Z. J. Li, "Optimal and consistent protein glycosylation in mammalian cell culture," Glycobiology, vol. 19, no. 9, pp. 936-949, 2009.
L. F. Bisson and C. E. Butzke, "Diagnosis and rectification of stuck and sluggish fermentations," Amer. J. Enology Viticulture, vol. 51, no. 2, pp. 168-177, 2000.
M. Bely, J.-M. Sablayrolles, and P. Barre, "Automatic detection of assimilable nitrogen deficiencies during alcoholic fermentation in oenological conditions," J. Fermentation Bioeng., vol. 70, no. 4, pp. 246-252, 1990.
M. Kudo, P. Vagnoli, and L. F. Bisson, "Imbalance of pH and potassium concentration as a cause of stuck fermentations," Amer. J. Enology Viticulture, vol. 49, no. 3, pp. 295-301, 1998.
É. D. Vieira, M. da Graça Stupiello Andrietta, and S. R. Andrietta, "Yeast biomass production: A new approach in glucose-limited feeding strategy," Brazilian J. Microbiol., vol. 44, no. 2, pp. 551-558, 2013.
G. Birol, P. Doruker, B. Kirdar, Z. I. Önsan, and K. Ülgen, "Mathematical description of ethanol fermentation by immobilised saccharomyces cerevisiae," Process Biochem., vol. 33, no. 7, pp. 763-771, 1998.
M. Koudelka, S. Gernet, and N. F. De Rooij, "Planar amperometric enzyme-based glucose microelectrode," Sens. Actuator, vol. 18, no. 2, pp. 157-165, 1989.
R. Vaidya and E. Wilkins, "Effect of interference on amperometric glucose biosensors with cellulose acetate membranes," Electroanalysis, vol. 6, no. 8, pp. 677-682, 1994.
D. J. Harrison, R. F. B. Turner, and H. P. Baltes, "Characterization of perfluorosulfonic acid polymer coated enzyme electrodes and a miniaturized integrated potentiostat for glucose analysis in whole blood," Anal. Chem., vol. 60, no. 19, pp. 2002-2007, 1988.
Y. Xu, A. S. Jeevarajan, J. M. Fay, T. D. Taylor, and M. M. Anderson, "Online measurement of glucose in a rotating wall perfused vessel bioreactor using an amperometric glucose sensor," J. Electrochem. Soc., vol. 149, no. 4, pp. H103-H106, 2002.
W. H. Mullen, F. H. Keedy, and S. J. Churchouse, "Glucose enzyme electrode with extended linearity: Application to undiluted blood measurements," Anal. Chim. Acta, vol. 183, pp. 59-66, May. 1986.
N. J. Ronkainen, H. B. Halsall, and W. R. Heineman, "Electrochemical biosensors," Chem. Soc. Rev., vol. 39, no. 5, pp. 1747-1763, 2010.
F.-G. Banica, Chemical Sensors and Biosensors: Fundamentals and Applications, 1st ed. Hoboken, NJ, USA: Wiley, 2012.
W. K. Ward, L. B. Jansen, E. Anderson, G. Reach, J.-C. Klein, and G. S. Wilson, "A new amperometric glucose microsensor: In vitro and short-term in vivo evaluation," Biosens. Bioelectron., vol. 17, no. 3, pp. 181-189, 2002.
A. P. Soldatkin et al., "Glucose sensitive conductometric biosensor with additional nafion membrane: Reduction of influence of buffer capacity on the sensor response and extension of its dynamic range," Anal. Chim. Acta, vol. 288, no. 3, pp. 197-203, 1994.
J. Zhou, A. V. Ellis, and N. H. Voelcker, "Recent developments in PDMS surface modification for microfluidic devices," Electrophoresis, vol. 31, no. 1, pp. 2-16, 2010.
W. Y. Zhang, G. S. Ferguson, and S. Tatic-Lucic, "Elastomer-supported cold welding for room temperature wafer-level bonding," in Proc. 17th IEEE Int. Conf. Micro Electro Mech. Syst., Jan. 2004, pp. 741-744.
J. H. Koschwanez, R. H. Carlson, and D. R. Meldrum, "Thin PDMS films using long spin times or tert-butyl alcohol as a solvent," PLoS One, vol. 4, no. 2, p. e4572, 2009.
W. Chen, R. H. W. Lam, and J. Fu, "Photolithographic surface micromachining of polydimethylsiloxane (PDMS)," Lab Chip, vol. 12, no. 2, pp. 391-395, 2012.
B. Krajewska and A. Olech, "Pore structure of gel chitosan membranes. I. Solute diffusion measurements," Polym. Gels Netw., vol. 4, no. 1, pp. 33-43, 1996.
C.-H. Lin, Y.-H. Yeh, W.-C. Lin, and M.-C. Yang, "Novel silicone hydrogel based on PDMS and PEGMA for contact lens application," Colloids Surf. B, Biointer., vol. 123, pp. 986-994, Nov. 2014.
V. Stepankova, S. Bidmanova, T. Koudelakova, Z. Prokop, R. Chaloupkova, and J. Damborsky, "Strategies for stabilization of enzymes in organic solvents," ACS Catal., vol. 3, no. 12, pp. 2823-2836, 2013.
A. T. Wolf, "Environmental degradation factors, their characterisation and effects on sealed building joints," Durability Building Sealants, RILEM, Bagneux, France, Tech Rep. 139-DBS, 1999.
R. Keshavaraj, R. W. Tock, and C. V. G. Vallabhan, "Effects of moisture on structural silicone rubber sealants used in window glazing applications," Construction Building Mater., vol. 8, no. 4, pp. 227-232, 1994.
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