Enzyme sensor with Polydimethylsiloxane membrane and CMOS potentiostat for wide-range glucose measurements.

[en] We report on an electrochemical measurement setup comprising a glucose sensor and a CMOS potentiostat with a two-layer membrane as the first steps toward the development of an integrated in-situ sensor system for bioreactors. The potentiostat has a chip size of 2.1 mm× 2.5 mm and a linear current range from −220 nA to 240 nA with a linearity of R2 = 0.9995. For wide range measurements of glucose concentrations in cell culture media, electrodes functionalized with the enzyme glucose oxidase were spin-coated with membranes made from polydimethylsiloxane (PDMS). A two-stage curing scheme of the PDMS was applied, and different membrane thicknesses and curing times were evaluated. With these membranes, glucose concentrations up to 500 mM were measured with a linear measurement range up to 200 mM. The sensors were successfully employed in the glucose monitoring of a culture of Saccharomyces cerevisiae to monitor the glucose consumption of the cells. For interference elimination, the cellulose acetate membranes were employed.

Disciplines :

Electrical & electronics engineering

Author, co-author :

Mross, Stefan

Fuerst, Peter

Pierrat, Sebastien

Vogt, Holger

Kraft, Michael ; Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes microélectroniques intégrés

Language :

English

Title :

Enzyme sensor with Polydimethylsiloxane membrane and CMOS potentiostat for wide-range glucose measurements.

Publication date :

2015

Journal title :

IEEE Sensors Journal

ISSN :

1530-437X

eISSN :

1558-1748

Publisher :

Institute of Electrical and Electronics Engineers, New York, United States - New York

Volume :

Issue :

Pages :

7096-7104

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 October 2015

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