Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements. - 2015
Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements.
Carnosine; Dietary supplements; Enantioseparation; Quinidine-based monolithic column; Small peptides
Résumé :
[en] In the present study, a new analytical methodology was developed enabling the enantiomeric determination of N-derivatized di- and tri-peptides in dietary supplements using chiral micro-LC on a monolithic column consisting of poly(O-9-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-co-2-hydroxy ethyl methacrylate-co-ethylene dimethacrylate) (poly(MQD-co-HEMA-co-EDMA)). After optimization of the mobile phase conditions, a baseline resolution of the stereoisomers of 24 out of 53 N-derivatized di- and tri-peptides was obtained. 3,5-Dinitrobenzoyl- and 3,5-dichlorobenzoyl-peptide stereoisomers were separated with exceptionally high selectivity and resolution. The monolithic column was then applied to the quantitative analysis of l-carnosine and its enantiomeric impurity in three different commercial dietary supplements. Method validation demonstrated satisfactory results in terms of linearity, precision, selectivity, accuracy and limits of detection and quantification. The determined amounts of l-carnosine in commercial formulations were in agreement with the labeled content for all analyzed samples, and the enantiomeric impurity was found to be below the limit of detection (LOD), showing the potential of the poly(MQD-co-HEMA-co-EDMA) monolithic column as a reliable tool for the quality control of l-carnosine in dietary supplements by micro-LC.
Centre/Unité de recherche :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacie, pharmacologie & toxicologie
Auteur, co-auteur :
Wang, Qiqin
Sanchez-Lopez, Elena
Han, Hai
Wu, Huihui
Zhu, Peijie
Crommen, Jacques ; Université de Liège > Département de pharmacie > Analyse des Médicaments
Marina, Maria Luisa
Jiang, Zhengjin
Langue du document :
Anglais
Titre :
Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements.
Date de publication/diffusion :
2015
Titre du périodique :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Maison d'édition :
Elsevier, Amsterdam, Pays-Bas
Peer reviewed :
Peer reviewed vérifié par ORBi
Commentaire :
Copyright (c) 2015 Elsevier B.V. All rights reserved.
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