Reference : A new method to identify the endangered Pyrenean desman (Galemys pyrenaicus) and to s...
Scientific journals : Article
Life sciences : Zoology
http://hdl.handle.net/2268/185886
A new method to identify the endangered Pyrenean desman (Galemys pyrenaicus) and to study its diet, using next generation sequencing from faeces
English
Gillet, François mailto [Université de Liège - ULiège > > > Form. doct. sc. (biol. orga. & écol. - Bologne)]
Tiouchichine, Marie-Laure [> >]
Galan, Maxime [> >]
Blanc, Frédéric [> >]
Némoz, Mélanie [> >]
Aulagnier, Stéphane [> >]
Michaux, Johan mailto [Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
2015
Mammalian Biology
Elsevier GmbH, Urban & Fischer Verlag
Yes (verified by ORBi)
1616-5047
[en] the Pyrenean Mountains and to the northern half of the Iberian Peninsula whose ecology and biology
are still poorly known. The aim of this study was to identify Pyrenean desman faeces and to analyze its
diet from this material using next-generation sequencing methods. We amplified and sequenced a small
DNA minibarcode (133 bp) of the COI gene in twenty-four putative faeces samples of Pyrenean desman
and successfully identified the species in 16 samples. Other identified species were mammals, birds and
amphibians, evidencing the potential application of our methods to a larger panel of taxa.
In the Pyrenean desman faeces, we were able to identify nineteen prey species with a positive match
(more than 98% of identity with a reference sequence) and eleven putative prey species with lower identity
scores (90–96%). The nineteen species belonged to four orders and eleven families among which
Trichoptera and Hydropsychidae were the most frequent, respectively. Future improvements could be
obtained by extending the reference DNA sequence collection to reach precise identifications over the
Desman’s range and by increasing the sampling to gain a better knowledge of the local diet of this endangered
species. Such information is of great importance to propose the best management measures for its
conservation.
http://hdl.handle.net/2268/185886
10.1016/j.mambio.2015.08.002

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