Reference : Quantification of hypoglycin A in serum using aTRAQ((R)) assay
Scientific journals : Article
Life sciences : Genetics & genetic processes
Quantification of hypoglycin A in serum using aTRAQ((R)) assay
BOEMER, François mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
DEBERG, Michelle mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
SCHOOS, Roland mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Baise, Etienne mailto [Université de Liège > Département de productions animales > Biostatistique, économie, sélection animale >]
Amory, Hélène mailto [Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés >]
Gault, Gilbert [> >]
Carlier, Jeremy [> >]
Gaillard, Yvan [> >]
Marcillaud-Pitel, Christel [> >]
Votion, Dominique mailto [Université de Liège > > Scientifiques attachés au Doyen (F MV) >]
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yes (verified by ORBi)
[en] Amino acids ; Atypical myopathy ; Hypoglycin A ; Mass spectrometry ; aTRAQ
[en] BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50mumol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
Fundamental and Applied Research for Animals and Health - FARAH
Researchers ; Professionals ; Students

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