Amino acids; Atypical myopathy; Hypoglycin A; Mass spectrometry; aTRAQ
Abstract :
[en] BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50mumol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
Research Center/Unit :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Genetics & genetic processes
Author, co-author :
BOEMER, François ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
DEBERG, Michelle ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
SCHOOS, Roland ; Centre Hospitalier Universitaire de Liège - CHU > Génétique
Baise, Etienne ; Université de Liège > Département de productions animales > Biostatistique, économie, sélection animale
Amory, Hélène ; Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés
Gault, Gilbert
Carlier, Jeremy
Gaillard, Yvan
Marcillaud-Pitel, Christel
Votion, Dominique ; Université de Liège > Scientifiques attachés au Doyen (F MV)
Language :
English
Title :
Quantification of hypoglycin A in serum using aTRAQ((R)) assay
Publication date :
2015
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Valberg S.J., Sponseller B.T., Hegeman A.D., Earing J., Bender J.B., Martinson K.L., et al. Seasonal pasture myopathy/atypical myopathy in North America associated with ingestion of hypoglycin a within seeds of the box elder tree. Equine. Vet. J. 2013, 45:419-426.
Votion D.M., van Galen G., Sweetman L., Boemer F., de Tullio P., Dopagne C., et al. Identification of methylenecyclopropyl acetic acid in serum of European horses with atypical myopathy. Equine. Vet. J. 2014, 46:146-149.
Unger L., Nicholson A., Jewitt E.M., Gerber V., Hegeman A., Sweetman L., Valberg S. Hypoglycin a concentrations in seeds of Acer pseudoplatanus trees growing on atypical myopathy-affected and control pastures. J. Vet. Intern. Med. 2014, 28:1289-1293.
Barceloux D.G. Akee fruit and Jamaican vomiting sickness (blighia sapida koenig). Dis. Mon. 2009, 55:318-326.
Ikeda Y., Tanaka K. Selective inactivation of various acyl-coa dehydrogenases by (methylenecyclopropyl) acetyl-coa. Biochim. Biophys. Acta. 1990, 1038:216-221.
Wenz A., Thorpe C., Ghisla S. Inactivation of general acyl-coa dehydrogenase from pig kidney by a metabolite of hypoglycin a. J. Biol. Chem. 1981, 256:9809-9812.
Gregersen N. The acyl-coa dehydrogenation deficiencies. Recent advances in the enzymic characterization and understanding of the metabolic and pathophysiological disturbances in patients with acyl-coa dehydrogenation deficiencies. Scand. J. Clin. Lab. Invest. Suppl. 1985, 174:1-60.
Tanaka K., Miller E.M., Isselbacher K.J. Hypoglycin a: a specific inhibitor of isovaleryl coa dehydrogenase. Proc. Natl. Acad. Sci. U. S. A. 1971, 68:20-24.
van der Kolk J.H., Wijnberg I.D., Westermann C.M., Dorland L., de Sain-van der Velden M.G., Kranenburg L.C., et al. Equine acquired multiple acyl-coa dehydrogenase deficiency (madd) in 14 horses associated with ingestion of maple leaves (Acer pseudoplatanus) covered with european tar spot (Rhytisma acerinum). Mol. Genet. Metab. 2010, 101:289-291.
Westermann C.M., Dorland L., Votion D.M., de Sain-van der Velden M.G., Wijnberg I.D., Wanders R.J., et al. Acquired multiple acyl-coa dehydrogenase deficiency in 10 horses with atypical myopathy. Neuromuscul. Disord. 2008, 18:355-364.
Filee R., Schoos R., Boemer F. Evaluation of physiological amino acids profiling by tandem mass spectrometry. JIMD Rep 2014, 13:119-128.
Carlier J., Guitton J., Moreau C., Boyer B., Bevalot F., Fanton L., et al A validated method for quantifying hypoglycin a in whole blood by uhplc-hrms/ms. J. Chromatogr. B 2014, 10.1016/j.jchromb.2014.11.029.
van Galen G., Marcillaud Pitel C., Saegerman C., Patarin F., Amory H., Baily J.D., et al. European outbreaks of atypical myopathy in grazing equids (2006-2009): spatiotemporal distribution, history and clinical features. Equine. Vet. J. 2012, 44:614-620.