Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.

[en] Primary cilia interpret vertebrate Hedgehog (Hh) signals. Why cilia are essential for signaling is unclear. One possibility is that some forms of signaling require a distinct membrane lipid composition, found at cilia. We found that the ciliary membrane contains a particular phosphoinositide, PI(4)P, whereas a different phosphoinositide, PI(4,5)P2, is restricted to the membrane of the ciliary base. This distribution is created by Inpp5e, a ciliary phosphoinositide 5-phosphatase. Without Inpp5e, ciliary PI(4,5)P2 levels are elevated and Hh signaling is disrupted. Inpp5e limits the ciliary level s of inhibitors of Hh signaling, including Gpr161 and the PI(4,5)2-binding protein Tulp3. Increasing ciliary PI(4,5)P2levels or conferring the ability to bind PI(4)P on Tulp3 increases the ciliary localization of Tulp3. Lowering Tulp3 in cells lacking Inpp5e

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Garcia-Gonzalo, F. R.

Phua, S. C.

Roberson, E. C.

Garcia III, G.

Abedin, M.

Schurmans, Stéphane ; Université de Liège > Département de sciences fonctionnelles (DSF) > Biochimie métabolique vétérinaire

Inoue, T

Reiter, J. F.

Language :

English

Title :

Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.

Publication date :

2015

Journal title :

Developmental Cell

ISSN :

1534-5807

Publisher :

Elsevier Science

Volume :

Pages :

400-409

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 August 2015

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