Neuron-Specific Enolase as a Marker of in Vitro Neuronal Damage. Part II: Investigation of the Astrocyte Protective Effect against Kainate-Induced Neurotoxicity
Bonhomme, Vincent; Hans, Pol; Collette, Julienet al.
1993 • In Journal of Neurosurgical Anesthesiology, 5 (2), p. 117-20
[en] The protective effect of astrocytes on hippocampal neurons against kainate-induced toxicity was investigated using neuron-specific enolase as an indicator of neuronal death. Astrocyte-rich and astrocyte-poor mixed rat hippocampal cultures were submitted to various concentrations of kainate. At each kainate concentration, the amount of NSE released by neurons was significantly greater in astrocyte-poor than in astrocyte-rich cultures (p < 0.05). This protective effect was not observed when neuronal survival was tested on astrocyte-poor cultures in astrocyte-conditioned culture medium with or without 10(-4) M kainate. In conclusion, astrocytes significantly attenuate kainate toxicity on hippocampal neurons, and this effect is not mediated by a diffusible factor.
Disciplines :
Anesthesia & intensive care
Author, co-author :
Bonhomme, Vincent ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Hans, Pol ; Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Collette, Julien ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Language :
English
Title :
Neuron-Specific Enolase as a Marker of in Vitro Neuronal Damage. Part II: Investigation of the Astrocyte Protective Effect against Kainate-Induced Neurotoxicity
Publication date :
April 1993
Journal title :
Journal of Neurosurgical Anesthesiology
ISSN :
0898-4921
eISSN :
1537-1921
Publisher :
Lippincott Williams & Wilkins, United States - Maryland