Spatio‑temporal dynamics of β‑tubulin isotypes during the development of the sensory auditory organ in rat

[en] There are different β-tubulin isoforms in microtubules of vertebrate tissues. However, their functional significance is still largely unknown. In the present study, we investigated the localization of five β-tubulin isotypes (β1– 5) within the hearing organ during development in rat. By using confocal microscopy, we showed that with the exception of the β3-tubulin isoform that was specific to nerve fibres, all the different β-tubulin isoforms were mainly present in the supporting cells. Contrary to β1–4-tubulins, we also found that the β5-tubulin isoform appeared only at a key stage of the post-natal development in specific cell types (pillar cells and Deiters’ cells). By using transmission electron microscopy, we revealed further that this developmental stage coincided with the formation of two separate bundles of microtubules from a unique one in these supporting cells. Together, these results suggest that the β5-tubulin isoform might be involved in the generation of new microtubule bundles from a pre-existing one.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Renauld, Justine ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Johnen, Nicolas

Thelen, Nicolas ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Cloes, Marie

Thiry, Marc ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Language :

English

Title :

Spatio‑temporal dynamics of β‑tubulin isotypes during the development of the sensory auditory organ in rat

Publication date :

26 July 2015

Journal title :

Histochemistry and Cell Biology

ISSN :

0948-6143

eISSN :

1432-119X

Publisher :

Springer Science & Business Media B.V., New York, United States - New York

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 July 2015

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