[en] Olfaction is crucial for short distance host location and pheromone detection by insects. Complexes of
olfactory receptors (ORs) are composed of odor-specific ORs and OR co-receptors (Orco). Orcos are widely
co-expressed with odor-specific ORs and are conserved across insect taxa. A number of Orco orthologs
have been studied to date, although none has been identified in cereal aphids. In this study, an Orco gene
ortholog was cloned from the grain aphid, Sitobion avenae, and named “SaveOrco”; RNA interference
(RNAi) reduced the expression of SaveOrco to 34.11% in aphids, resulting in weaker EAG (electroantennogram)
responses to plant volatiles (Z-3-hexene-1-ol; methyl salicylate, MeSA) and aphid alarm
pheromone (E-b-farnesene, EBF). Aphid wing differentiation induced by EBF was investigated in both
RNAi treated and untreated aphids. EBF induced production of winged aphids in both pre-natal and postnatal
periods in untreated aphids, but no such induction was observed in the RNAi-treated aphids. We
conclude that SaveOrco is crucial for the aphid's response to pheromones and other volatiles, and is
involved in wing differentiation triggered by EBF
Disciplines :
Entomology & pest control
Author, co-author :
Fan, Jia; Chinese Academy of Agricultural Sciences > Institute of Plant Protection > State Key Laboratory of Plant Diseases and Insect Pests
Zhang, Yong ; Chinese Academy of Agricultural Sciences > Institute of Plant Protection > State Key Laboratory of Plant Diseases and Insect Pests
Francis, Frédéric ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
Cheng, Dengfa; Chinese Academy of Agricultural Sciences > Institute of Plant Protection > State Key Laboratory of Plant Diseases and Insect Pests
Sun, Jingrun; Chinese Academy of Agricultural Sciences > Institute of Plant Protection > State Key Laboratory of Plant Diseases and Insect Pests
Chen, Julian; Chinese Academy of Agricultural Sciences > Institute of Plant Protection > State Key Laboratory of Plant Diseases and Insect Pests > chenjulian@caas.cn
Language :
English
Title :
Orco mediates olfactory behaviors and winged morph differentiation induced by alarm pheromone in the grain aphid, Sitobion avenae
Publication date :
27 May 2015
Journal title :
Insect Biochemistry and Molecular Biology
ISSN :
0965-1748
eISSN :
1879-0240
Publisher :
Pergamon Press (part of Elsevier Science), Oxford, United Kingdom
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