[en] Poly(2-dimethylamino)ethyl methacrylate (PDMAEMA) is an attractive polycation frequently proposed as a non-viral vector for gene therapy. As expected for other cationic carriers, intravenous administration of PDMAEMA can result in its ionic complexation with various negatively charged domains found within the blood. To gain more insight into this polycation hemoreactivity, we followed the binding kinetics of a free form (FF) of fluorescein labelled PDMAEMA (below 15 kDa) in normal human blood using flow cytometry. This in vitro study highlighted that platelets display higher affinity for this polycation compared to red blood cells (RBCs), with an adsorption isotherm characteristics of a specific saturable binding site. PDMAEMA (1-20μg/mL) exerted a concentration dependent proaggregant effect with a biphasic aggregation of washed platelets. Activation of platelets was also noticed in whole blood with the expression of P-selectin and fibrinogen on platelet surface. Although additional studies would be needed in order to elucidate the mechanism of PDMAEMA mediated activation of platelets, our manuscript provides important information on the hemoreactivity of FF PDMAEMA.
Research center :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Chemistry
Author, co-author :
Flebus, Luca ; Université de Liège > Centre interfacultaire des biomatériaux (CEIB)
Lombart, François ; Université de Liège > Centre interfacultaire des biomatériaux (CEIB)
Martinez-Jothar, L
Sevrin, Chantal ; Université de Liège > Centre interfacultaire des biomatériaux (CEIB)
Delierneux, Céline ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-Sciences Cardiovasculaires
Oury, Cécile ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-Sciences Cardiovasculaires
Grandfils, Christian ; Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
In vitro study of the specific interaction between poly (2-dimethylamino ethylmethacrylate) based polymers with platelets and red blood cells.
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