Article (Scientific journals)
Identification of the neutralizing epitopes of Merkel cell polyomavirus major capsid protein within the BC and EF surface loops.
Fleury, Maxime J. J.; Nicol, Jérôme; Samimi, Mahtab et al.
2015In PLoS ONE, 10 (3), p. 0121751
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Abstract :
[en] Merkel cell polyomavirus (MCPyV) is the first polyomavirus clearly associated with a human cancer, i.e. the Merkel cell carcinoma (MCC). Polyomaviruses are small naked DNA viruses that induce a robust polyclonal antibody response against the major capsid protein (VP1). However, the polyomavirus VP1 capsid protein epitopes have not been identified to date. The aim of this study was to identify the neutralizing epitopes of the MCPyV capsid. For this goal, four VP1 mutants were generated by insertional mutagenesis in the BC, DE, EF and HI loops between amino acids 88-89, 150-151, 189-190, and 296-297, respectively. The reactivity of these mutants and wild-type VLPs was then investigated with anti-VP1 monoclonal antibodies and anti-MCPyV positive human sera. The findings together suggest that immunodominant conformational neutralizing epitopes are present at the surface of the MCPyV VLPs and are clustered within BC and EF loops.
Disciplines :
Microbiology
Author, co-author :
Fleury, Maxime J. J.
Nicol, Jérôme ;  Université de Liège > Département des sciences biomédicales et précliniques > Virologie
Samimi, Mahtab
Arnold, Francoise
Cazal, Raphael
Ballaire, Raphaelle
Mercey, Olivier
Gonneville, Helene
Combelas, Nicolas
Vautherot, Jean-Francois
Moreau, Thierry
Lorette, Gerard
Coursaget, Pierre
Touze, Antoine
More authors (4 more) Less
Language :
English
Title :
Identification of the neutralizing epitopes of Merkel cell polyomavirus major capsid protein within the BC and EF surface loops.
Publication date :
2015
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
10
Issue :
3
Pages :
e0121751
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 June 2015

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