Article (Scientific journals)
Determination of the singlet oxygen quantum yield of bacteriochlorin a: a comparative study in phosphate buffer and aqueous dispersion of dimiristoyl-L-a-phosphatidylcholine liposomes
Hoebeke, Maryse; Damoiseau, Xavier
2002In Photochemical and Photobiological Sciences, 1 (4), p. 283-287
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Keywords :
Photodynamic therapy; bacterichlorin; liposome
Abstract :
[en] The production of singlet oxygen (1O2) by bacteriochlorin a (BCA) was studied in phosphate buffer and in dimyristoyl-L-α-phosphatidylcholine (DMPC) unilamellar liposomes. The comparative method used to measure 1O2 production was a quantitative analysis of photooxidation reactions leading to the loss of absorbance of the water-soluble specific probe: anthracene 9,10-dipropionic acid. Rose Bengal, whose 1O2 quantum yield (ΦRB) is well known in alcohols and phosphate buffer, was used as the standard for the quantification of the BCA singlet oxygen production. Our results confirm quantitatively that solubilization of BCA in liposomes leads to an increase in 1O2 production. Indeed, the quantum yield of 1O2 production by BCA (ΦBCA) is 0.05 in phosphate buffer and 0.33 in DMPC liposomes. Furthermore, the diffusion characteristics of 1O2 produced by BCA bound to liposome were also examined using the isotopic lifetime enhancement effect of D2O. It was shown that 1O2 spent at least 70% of its lifetime in the vesicular environment
Disciplines :
Physics
Author, co-author :
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Damoiseau, Xavier
Language :
English
Title :
Determination of the singlet oxygen quantum yield of bacteriochlorin a: a comparative study in phosphate buffer and aqueous dispersion of dimiristoyl-L-a-phosphatidylcholine liposomes
Publication date :
April 2002
Journal title :
Photochemical and Photobiological Sciences
ISSN :
1474-905X
eISSN :
1474-9092
Publisher :
Royal Society of Chemistry, Cambridge, United Kingdom
Volume :
1
Issue :
4
Pages :
283-287
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 April 2010

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