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Towards the identification of cis-elements responsible for the high expression of HMA4, an essential gene for Zn hyperaccumulation in Arabidopsis halleri.
Spielmann, Julien; Nouet, Cécile; Scheepers, Maxime et al.
2015VISCEA - Plant Growth, Nutrition & Environment Interaction
 

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Keywords :
Arabidopsis halleri; HMA4; zinc hyperaccumulation
Abstract :
[en] A. halleri is a Zn and Cd hypertolerant and hyperaccumulator plant which stores metals in shoot vacuoles. One key step for hyperaccumulation is a highly efficient root-to-shoot translocation of metals. The Zn/Cd plasmamembrane pump HMA4 (heavy metal ATPase 4) plays a central role in this process and is involved in xylem loading/unloading. Moreover, HMA4 is overexpressed in A. halleri compare to its non-tolerant and non-hyperaccumulator sister, Arabidopsis thaliana. The HMA4 overexpression is determined by a combination of gene triplication and cis-regulatory changes increasing transcription levels of each three HMA4 copies. In this study, we are focusing on the two most active HMA4 promoters and we aim to identify the cis-elements underlying high expression of HMA4 in A. halleri using promoter-reporter constructs. Through deletion analysis, two important regions for a high expression have been identified. Ongoing analysis of more detailed deletions will be presented.
Disciplines :
Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Spielmann, Julien ;  Université de Liège - ULiège > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)
Nouet, Cécile  ;  Université de Liège > Département des sciences de la vie > Département des sciences de la vie
Scheepers, Maxime ;  Université de Liège - ULiège > Form. doct. sc. (bioch., biol. mol. cel., bioinf. - paysage)
Krämer, Ute
Motte, Patrick ;  Université de Liège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Hanikenne, Marc  ;  Université de Liège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Language :
English
Title :
Towards the identification of cis-elements responsible for the high expression of HMA4, an essential gene for Zn hyperaccumulation in Arabidopsis halleri.
Publication date :
26 June 2015
Event name :
VISCEA - Plant Growth, Nutrition & Environment Interaction
Event place :
Vienne, Austria
Event date :
25-26 juin 2015
By request :
Yes
Audience :
International
Available on ORBi :
since 10 June 2015

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