Poster (Scientific congresses and symposiums)
Towards the identification of mechanisms underlying the high expression of HMA4, an essential gene for zinc hyperaccumulation in Arabidopsis halleri.
Spielmann, Julien; Nouet, Cécile; Scheepers, Maxime et al.
2015IAP meeting
 

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Keywords :
Arabidopsis halleri; HMA4; zinc hyperaccumulation
Abstract :
[en] To maintain metal homeostasis within physiological limits independently of metal concentrations present in soil, plants developed a complex network including metal uptake, chelation, trafficking, and storage processes. In this network, transporters have a central role. To study the molecular basis of zinc homeostasis in plants, we are using A. halleri, a zinc- and cadmium-tolerant and zinc-hyperaccumulating species representing the extreme end of natural variation in terms of metal homeostasis. Indeed, A. halleri stores high amount of metals in vacuoles of above-ground tissues (>1% Zn or 0.01% Cd in shoot dry weight). Cross-species transcriptomic studies identified about thirty genes which are constitutively over-expressed in A. halleri compared to its non-tolerant and non-hyperaccumulator sister species, Arabidopsis thaliana (1-3). HMA4 is one of these genes. It encodes a Zn/Cd plasmamembrane pump involved in xylem loading/unloading which is a key step in the hyperaccumulation process (4-5). The HMA4 overexpression is determined by a combination of gene triplication and cis-regulatory changes increasing transcription levels of each three HMA4 copies (5). We aim to identify the cis-elements underlying high expression of HMA4 in A. halleri using promoter-reporter constructs.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Biochemistry, biophysics & molecular biology
Author, co-author :
Spielmann, Julien ;  Université de Liège - ULiège > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)
Nouet, Cécile  ;  Université de Liège > Département des sciences de la vie > Département des sciences de la vie
Scheepers, Maxime ;  Université de Liège - ULiège > Form. doct. sc. (bioch., biol. mol. cel., bioinf. - paysage)
Motte, Patrick ;  Université de Liège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Krâmer, Ute
Hanikenne, Marc  ;  Université de Liège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Language :
English
Title :
Towards the identification of mechanisms underlying the high expression of HMA4, an essential gene for zinc hyperaccumulation in Arabidopsis halleri.
Publication date :
08 June 2015
Event name :
IAP meeting
Event organizer :
ULg - Université de Liège
Event place :
Liège, Belgium
Event date :
08 juin 2015
Funders :
ULiège - Université de Liège [BE]
Available on ORBi :
since 09 June 2015

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