Keywords :
14-3-3 Proteins; Adult; Aged; Astrocytoma/cerebrospinal fluid/diagnosis; Biological Markers/cerebrospinal fluid; Brain Diseases/cerebrospinal fluid/diagnosis; Brain Neoplasms/cerebrospinal fluid/diagnosis; Cerebrospinal Fluid/chemistry; Creutzfeldt-Jakob Syndrome/cerebrospinal fluid/diagnosis; Diagnosis, Differential; False Positive Reactions; Female; Humans; Lymphoma/cerebrospinal fluid/diagnosis; Male; Middle Aged; Predictive Value of Tests; Prospective Studies; Proteins/analysis; Sensitivity and Specificity; Tyrosine 3-Monooxygenase
Abstract :
[en] OBJECTIVE: To study the sensitivity and specificity of 14-3-3 testing in a prospective series of patients suspected of having Creutzfeldt-Jakob disease (CJD). BACKGROUND: The 14-3-3 protein immunoassay on CSF has favorable test characteristics as a premortem diagnostic tool in CJD. However, the 14-3-3 protein is a normal cellular protein expressed in various tissues, and its presence in CSF reflects extensive destruction of brain tissue as in CJD, but also in ischemic stroke and meningoencephalitis. METHODS: 14-3-3 was tested in the CSF of a prospective series of 110 consecutive patients suspected of having CJD. RESULTS: The sensitivity was 97% and the specificity was 87% in this series. False-positive results were mainly caused by stroke and meningoencephalitis. CONCLUSION: The 14-3-3 protein is a highly sensitive and specific marker for CJD when used in the appropriate clinical context.
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