Reference : HYDRODYNAMICS IN A DISPOSABLE RECTANGULAR PARALLELEPIPED STIRRED BIOREACTOR WITH ELL...
Scientific journals : Article
Engineering, computing & technology : Chemical engineering
http://hdl.handle.net/2268/179819
HYDRODYNAMICS IN A DISPOSABLE RECTANGULAR PARALLELEPIPED STIRRED BIOREACTOR WITH ELLIPTIC PENDULUM MOTION PADDLE
English
Collignon, Marie-Laure mailto [Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée >]
Droissart, Laurent [Université de Liège - ULiège > Chimie Appliquée > Laboratoire de Génie Chimique > >]
Delafosse, Angélique mailto [Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques >]
Calvo, Sébastien mailto [Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Nanomatériaux et interfaces >]
Vanhamel, Steven [ATMI LifeSciences > > > >]
Rodriguez, Roman [ATMI LifeSciences > > > >]
Claes, Tom [ATMI LifeSciences > > > >]
Moncaubeig, Fabien [Artelis > > > >]
Peeters, Ludovic [GSK Vaccines > > > >]
Crine, Michel mailto [Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée >]
Toye, Dominique mailto [Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques >]
15-Jan-2015
Biochemical Engineering Journal
Elsevier Science
93
Yes (verified by ORBi)
International
1369-703X
Lausanne
Switzerland
[en] Agitation ; animal cell culture ; bioprocess design ; fluid mechanics ; stereo-PIV ; mean flow characterization
[en] Stainless steel bioreactors increasingly fall behind to their disposable counterparts in pharma research as they do not require cleaning or sterilization. This led company ATMI LifeSciences to develop the “Nucleo™”. Original in design, this disposable bioreactor comprises a rectangular parallelepiped plastic bag stirred by a paddle revolving in elliptic pendulum motion. Studies covering this bioreactor showed good homogeneity of culture medium as well as good productivity for animal cell cultures. To further explain these good performances, the flow inside the “Nucleo™” had to be resolved. This paper focuses on the mean flow description, computed from stereo-PIV measurements performed in 20 verticals covering the whole volume of a 50 dm3 Nucleo™ bioreactor. As the flow is already turbulent in the chosen agitation conditions, its dimensionless mean velocity field does not vary with the paddle rotational speed. Mean flow pattern exhibits an axial symmetry – same flow is observed in opposite quarters of the tank – and can be described as a three-dimensional helix coiled on itself to form a distorted horizontal torus which covers the whole tank volume. Mean velocity value is on average doubled in the cone swept by the paddle, and its two horizontal components are twice higher than its vertical ones. However, mean velocity remains significant everywhere and, in particular, no stagnant area is observed in tank corners. Our results thus confirm previous studies observations.
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Développement d'un modèle hydrodynamique multi-échelle décrivant le mélange dans les bioréacteurs utilisés en culture cellulaire. Validation expérimentale par les techniques de visualisation PIV et LIF.
http://hdl.handle.net/2268/179819
10.1016/j.bej.2014.10.011
[fr] http://reflexions.ulg.ac.be/ReacteursKleenex
[en] http://reflexions.ulg.ac.be/en/KleenexBioreactors

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